Results 1 - 11 of 11 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
17607 | 5' | -56.3 | NC_004466.2 | + | 34573 | 0.66 | 0.591552 |
Target: 5'- uGGCAucCCACCagaccAGGGCAGCAGcacCCGa -3' miRNA: 3'- gUCGU--GGUGGa----UCCUGUCGUCa--GGUc -5' |
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17607 | 5' | -56.3 | NC_004466.2 | + | 35458 | 0.66 | 0.569319 |
Target: 5'- -cGUGCUgacaGCC-AGGGCAGCAGccgCCAGa -3' miRNA: 3'- guCGUGG----UGGaUCCUGUCGUCa--GGUC- -5' |
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17607 | 5' | -56.3 | NC_004466.2 | + | 20191 | 0.66 | 0.547298 |
Target: 5'- gCAGUguugacaacguuACCACCcAGGugAGUAGUCacaAGg -3' miRNA: 3'- -GUCG------------UGGUGGaUCCugUCGUCAGg--UC- -5' |
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17607 | 5' | -56.3 | NC_004466.2 | + | 1038 | 0.67 | 0.492531 |
Target: 5'- cUAGCcCCucCCUAGGAUaccauaaGGCuAGUCCGGg -3' miRNA: 3'- -GUCGuGGu-GGAUCCUG-------UCG-UCAGGUC- -5' |
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17607 | 5' | -56.3 | NC_004466.2 | + | 42989 | 0.67 | 0.483121 |
Target: 5'- gUAGCGCCGCCUAGGGaAGCcauGUUg-- -3' miRNA: 3'- -GUCGUGGUGGAUCCUgUCGu--CAGguc -5' |
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17607 | 5' | -56.3 | NC_004466.2 | + | 40695 | 0.68 | 0.472769 |
Target: 5'- -cGCGaCACCUGGGGCaAGCuGUgCCGGg -3' miRNA: 3'- guCGUgGUGGAUCCUG-UCGuCA-GGUC- -5' |
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17607 | 5' | -56.3 | NC_004466.2 | + | 19429 | 0.68 | 0.472769 |
Target: 5'- gUAGCugCACCUGcGACAG-GGcCCAGg -3' miRNA: 3'- -GUCGugGUGGAUcCUGUCgUCaGGUC- -5' |
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17607 | 5' | -56.3 | NC_004466.2 | + | 19635 | 0.68 | 0.462529 |
Target: 5'- aGGCucgGCCACCUGGGcCGGCuGcUUCAGu -3' miRNA: 3'- gUCG---UGGUGGAUCCuGUCGuC-AGGUC- -5' |
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17607 | 5' | -56.3 | NC_004466.2 | + | 14262 | 0.68 | 0.462529 |
Target: 5'- aGGCACCACCcAGGGCaccaagGGCAGaaCCGa -3' miRNA: 3'- gUCGUGGUGGaUCCUG------UCGUCa-GGUc -5' |
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17607 | 5' | -56.3 | NC_004466.2 | + | 38111 | 0.68 | 0.452407 |
Target: 5'- -uGCGCagGCCacgAGGACAGCAaccacgggGUCCAGu -3' miRNA: 3'- guCGUGg-UGGa--UCCUGUCGU--------CAGGUC- -5' |
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17607 | 5' | -56.3 | NC_004466.2 | + | 17046 | 1.09 | 0.000508 |
Target: 5'- cCAGCACCACCUAGGACAGCAGUCCAGg -3' miRNA: 3'- -GUCGUGGUGGAUCCUGUCGUCAGGUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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