Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 17609 | 3' | -56.4 | NC_004466.2 | + | 13637 | 0.66 | 0.588845 |
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Target: 5'- gAGCCCUCcagUCucuCCuggguaGCUCAggUGUUAGCUCu -3' miRNA: 3'- -UCGGGAG---AGu--GG------UGAGU--ACAGUCGGG- -5' |
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| 17609 | 3' | -56.4 | NC_004466.2 | + | 43196 | 0.68 | 0.4312 |
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Target: 5'- uGGCCgUCUCACCGaUCAcuUC-GCCCu -3' miRNA: 3'- -UCGGgAGAGUGGUgAGUacAGuCGGG- -5' |
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| 17609 | 3' | -56.4 | NC_004466.2 | + | 38454 | 0.69 | 0.421543 |
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Target: 5'- cAGCCCgcuUCACCAUUCcUGUCcucgaAGUCCc -3' miRNA: 3'- -UCGGGag-AGUGGUGAGuACAG-----UCGGG- -5' |
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| 17609 | 3' | -56.4 | NC_004466.2 | + | 26451 | 0.7 | 0.36645 |
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Target: 5'- cGGCCCUCgUGCCACcaAUGaUCuGCCCa -3' miRNA: 3'- -UCGGGAGaGUGGUGagUAC-AGuCGGG- -5' |
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| 17609 | 3' | -56.4 | NC_004466.2 | + | 22214 | 0.71 | 0.322074 |
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Target: 5'- uGCCUUCUCccacuacuucuauaACCccaACUCcgGUCGGCCUu -3' miRNA: 3'- uCGGGAGAG--------------UGG---UGAGuaCAGUCGGG- -5' |
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| 17609 | 3' | -56.4 | NC_004466.2 | + | 17605 | 1.11 | 0.000369 |
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Target: 5'- aAGCCCUCUCACCACUCAUGUCAGCCCu -3' miRNA: 3'- -UCGGGAGAGUGGUGAGUACAGUCGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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