Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1761 | 5' | -62.6 | NC_001347.2 | + | 2665 | 0.7 | 0.490489 |
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Target: 5'- --gGCCa-CGGUgguuaUGGaGGCCGGCGGCc -3' miRNA: 3'- gcaCGGagGCCA-----ACCaCCGGCCGCCG- -5' |
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| 1761 | 5' | -62.6 | NC_001347.2 | + | 78344 | 0.71 | 0.464293 |
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Target: 5'- gCGUGguCCgggcggacCCGGcgUGGUGGgacCCGGCGGCg -3' miRNA: 3'- -GCAC--GGa-------GGCCa-ACCACC---GGCCGCCG- -5' |
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| 1761 | 5' | -62.6 | NC_001347.2 | + | 148606 | 0.72 | 0.406194 |
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Target: 5'- gCGUGaCCggCGGUgcggucgcgGGUGGCCGGCGucaGCg -3' miRNA: 3'- -GCAC-GGagGCCAa--------CCACCGGCCGC---CG- -5' |
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| 1761 | 5' | -62.6 | NC_001347.2 | + | 66779 | 0.72 | 0.382731 |
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Target: 5'- aCGUccGCCcgaCGG-UGGUGGCggCGGCGGCa -3' miRNA: 3'- -GCA--CGGag-GCCaACCACCG--GCCGCCG- -5' |
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| 1761 | 5' | -62.6 | NC_001347.2 | + | 112630 | 0.73 | 0.344252 |
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Target: 5'- uCGcGCCgacgguaauaggCCGGUgaUGGUGG-CGGCGGCa -3' miRNA: 3'- -GCaCGGa-----------GGCCA--ACCACCgGCCGCCG- -5' |
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| 1761 | 5' | -62.6 | NC_001347.2 | + | 229674 | 0.74 | 0.298257 |
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Target: 5'- uGUGCCggCGGggugUGGUGGCgggguguguCGGCGGUg -3' miRNA: 3'- gCACGGagGCCa---ACCACCG---------GCCGCCG- -5' |
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| 1761 | 5' | -62.6 | NC_001347.2 | + | 195 | 0.74 | 0.298257 |
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Target: 5'- uGUGCCggCGGggugUGGUGGCgggguguguCGGCGGUg -3' miRNA: 3'- gCACGGagGCCa---ACCACCG---------GCCGCCG- -5' |
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| 1761 | 5' | -62.6 | NC_001347.2 | + | 182221 | 0.76 | 0.250419 |
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Target: 5'- gCGUcGCCUUCGGUgacgucggUGGUGGCUGuaGGCu -3' miRNA: 3'- -GCA-CGGAGGCCA--------ACCACCGGCcgCCG- -5' |
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| 1761 | 5' | -62.6 | NC_001347.2 | + | 47779 | 1.11 | 0.000923 |
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Target: 5'- cCGUGCCUCCGGUUGGUGGCCGGCGGCa -3' miRNA: 3'- -GCACGGAGGCCAACCACCGGCCGCCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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