Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 1761 | 5' | -62.6 | NC_001347.2 | + | 13996 | 0.66 | 0.723 |
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Target: 5'- aCGUcUCUCCGGUaacuaucGGcGGCCGG-GGCu -3' miRNA: 3'- -GCAcGGAGGCCAa------CCaCCGGCCgCCG- -5' |
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| 1761 | 5' | -62.6 | NC_001347.2 | + | 2083 | 0.67 | 0.695258 |
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Target: 5'- uGUGCCUCCGcagccguacGGcgccGCCGGCGGg -3' miRNA: 3'- gCACGGAGGCcaa------CCac--CGGCCGCCg -5' |
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| 1761 | 5' | -62.6 | NC_001347.2 | + | 139310 | 0.67 | 0.68591 |
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Target: 5'- gCGUGCCUaCCGaucUGGcGGCguugugcgCGGCGGUg -3' miRNA: 3'- -GCACGGA-GGCca-ACCaCCG--------GCCGCCG- -5' |
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| 1761 | 5' | -62.6 | NC_001347.2 | + | 173706 | 0.67 | 0.694325 |
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Target: 5'- gGUGgUUCCGGUacgcggaaaaUgagcagcGGUGG-CGGCGGCg -3' miRNA: 3'- gCACgGAGGCCA----------A-------CCACCgGCCGCCG- -5' |
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| 1761 | 5' | -62.6 | NC_001347.2 | + | 192179 | 0.67 | 0.667108 |
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Target: 5'- --cGUCUCCGGaUGaGcGGCCG-CGGCg -3' miRNA: 3'- gcaCGGAGGCCaAC-CaCCGGCcGCCG- -5' |
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| 1761 | 5' | -62.6 | NC_001347.2 | + | 2127 | 0.67 | 0.657666 |
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Target: 5'- gCGUGCCUaacgCGGacgcgcacgcGGUGGUCGG-GGCg -3' miRNA: 3'- -GCACGGAg---GCCaa--------CCACCGGCCgCCG- -5' |
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| 1761 | 5' | -62.6 | NC_001347.2 | + | 30606 | 0.67 | 0.667108 |
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Target: 5'- -cUGCggCCGGccgcGGU-GCCGGCGGCu -3' miRNA: 3'- gcACGgaGGCCaa--CCAcCGGCCGCCG- -5' |
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| 1761 | 5' | -62.6 | NC_001347.2 | + | 152384 | 0.67 | 0.657666 |
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Target: 5'- gCGUcGCCUgcgaCGGcgGGUGGagaugaGGCGGCg -3' miRNA: 3'- -GCA-CGGAg---GCCaaCCACCgg----CCGCCG- -5' |
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| 1761 | 5' | -62.6 | NC_001347.2 | + | 47779 | 1.11 | 0.000923 |
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Target: 5'- cCGUGCCUCCGGUUGGUGGCCGGCGGCa -3' miRNA: 3'- -GCACGGAGGCCAACCACCGGCCGCCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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