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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 17610 | 3' | -55.4 | NC_004466.2 | + | 36918 | 0.66 | 0.645657 |
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Target: 5'- gGCGUcaucagGCCGCCuUCGACUACcacggaguaAGCCUUg -3' miRNA: 3'- -UGUA------UGGCGG-AGCUGGUGa--------UCGGAGu -5' |
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| 17610 | 3' | -55.4 | NC_004466.2 | + | 17371 | 0.67 | 0.578452 |
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Target: 5'- cCAUACCGauccuaacgccCCUCaGGCUGCUccuacGGCCUCAg -3' miRNA: 3'- uGUAUGGC-----------GGAG-CUGGUGA-----UCGGAGU- -5' |
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| 17610 | 3' | -55.4 | NC_004466.2 | + | 6124 | 0.67 | 0.55632 |
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Target: 5'- ---aAUUGCCUUGGCUugUagGGCCUCGg -3' miRNA: 3'- uguaUGGCGGAGCUGGugA--UCGGAGU- -5' |
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| 17610 | 3' | -55.4 | NC_004466.2 | + | 15397 | 0.69 | 0.47088 |
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Target: 5'- gGCAUca-GCCUCGACUAC-AGCCUgGg -3' miRNA: 3'- -UGUAuggCGGAGCUGGUGaUCGGAgU- -5' |
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| 17610 | 3' | -55.4 | NC_004466.2 | + | 17810 | 1.09 | 0.000747 |
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Target: 5'- gACAUACCGCCUCGACCACUAGCCUCAg -3' miRNA: 3'- -UGUAUGGCGGAGCUGGUGAUCGGAGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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