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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
17612 | 3' | -58.9 | NC_004466.2 | + | 20059 | 1.08 | 0.000362 |
Target: 5'- gUGCCACCGUUCUGGUCAUCCGGGCCAa -3' miRNA: 3'- -ACGGUGGCAAGACCAGUAGGCCCGGU- -5' |
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17612 | 3' | -58.9 | NC_004466.2 | + | 32413 | 0.7 | 0.265751 |
Target: 5'- cGCCuaCGUUCUGGagaUCAacaccgcUCCGGGCCu -3' miRNA: 3'- aCGGugGCAAGACC---AGU-------AGGCCCGGu -5' |
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17612 | 3' | -58.9 | NC_004466.2 | + | 40789 | 0.71 | 0.222438 |
Target: 5'- cGCCGUCGUUguaCUGGUCAgCCaGGGCCu -3' miRNA: 3'- aCGGUGGCAA---GACCAGUaGG-CCCGGu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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