Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 17614 | 5' | -50.5 | NC_004466.2 | + | 19811 | 0.66 | 0.895425 |
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Target: 5'- -aCACCCUCAUCGGUGccccuggugagacuUCuggugguaacGUaGACCCa -3' miRNA: 3'- uaGUGGGAGUAGUUAU--------------AG----------CGaCUGGG- -5' |
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| 17614 | 5' | -50.5 | NC_004466.2 | + | 38265 | 0.66 | 0.87422 |
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Target: 5'- --gACCCaUCAUCAAUGcccugCGgaUGGCCCa -3' miRNA: 3'- uagUGGG-AGUAGUUAUa----GCg-ACUGGG- -5' |
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| 17614 | 5' | -50.5 | NC_004466.2 | + | 44646 | 0.66 | 0.87422 |
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Target: 5'- cUgGCCCUUGUCGAUGgcaCGCUGgAUCUu -3' miRNA: 3'- uAgUGGGAGUAGUUAUa--GCGAC-UGGG- -5' |
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| 17614 | 5' | -50.5 | NC_004466.2 | + | 22402 | 0.66 | 0.865843 |
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Target: 5'- cAUUACCCgcuUgGAUAUCGCUgagGACUCg -3' miRNA: 3'- -UAGUGGGaguAgUUAUAGCGA---CUGGG- -5' |
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| 17614 | 5' | -50.5 | NC_004466.2 | + | 17411 | 0.66 | 0.865843 |
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Target: 5'- --aGCCUgcugCAUCcuUGUUGCUGGCCa -3' miRNA: 3'- uagUGGGa---GUAGuuAUAGCGACUGGg -5' |
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| 17614 | 5' | -50.5 | NC_004466.2 | + | 22652 | 0.67 | 0.857194 |
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Target: 5'- -aUACCCUCGUCA---UUGCggauACCCa -3' miRNA: 3'- uaGUGGGAGUAGUuauAGCGac--UGGG- -5' |
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| 17614 | 5' | -50.5 | NC_004466.2 | + | 30482 | 0.67 | 0.820072 |
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Target: 5'- aGUCGCUaugacaCUCAagAAUAUCGCggguaaggUGGCCCa -3' miRNA: 3'- -UAGUGG------GAGUagUUAUAGCG--------ACUGGG- -5' |
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| 17614 | 5' | -50.5 | NC_004466.2 | + | 30537 | 0.68 | 0.810213 |
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Target: 5'- -cCACCUaCAccaacgauuUCAccGUcgCGCUGACCCa -3' miRNA: 3'- uaGUGGGaGU---------AGU--UAuaGCGACUGGG- -5' |
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| 17614 | 5' | -50.5 | NC_004466.2 | + | 40634 | 0.69 | 0.73608 |
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Target: 5'- cUUGCCC-CA--GGUGUCGCgGACCCg -3' miRNA: 3'- uAGUGGGaGUagUUAUAGCGaCUGGG- -5' |
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| 17614 | 5' | -50.5 | NC_004466.2 | + | 23340 | 1.1 | 0.001922 |
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Target: 5'- gAUCACCCUCAUCAAUAUCGCUGACCCc -3' miRNA: 3'- -UAGUGGGAGUAGUUAUAGCGACUGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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