Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
17619 | 5' | -56.3 | NC_004466.2 | + | 40687 | 0.68 | 0.471077 |
Target: 5'- cUGGGGCA--AGCUGUgCC-GGGAAa- -3' miRNA: 3'- -ACCCCGUucUCGAUAgGGaCCCUUcc -5' |
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17619 | 5' | -56.3 | NC_004466.2 | + | 14289 | 0.73 | 0.251547 |
Target: 5'- cGGGGCcuccgAAGAGUgcUCCCaGGGAGGc -3' miRNA: 3'- aCCCCG-----UUCUCGauAGGGaCCCUUCc -5' |
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17619 | 5' | -56.3 | NC_004466.2 | + | 986 | 0.77 | 0.127041 |
Target: 5'- aGGGGCuAGGGGCaUAcCCCUGGGGauAGGc -3' miRNA: 3'- aCCCCG-UUCUCG-AUaGGGACCCU--UCC- -5' |
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17619 | 5' | -56.3 | NC_004466.2 | + | 30661 | 1.1 | 0.000516 |
Target: 5'- gUGGGGCAAGAGCUAUCCCUGGGAAGGc -3' miRNA: 3'- -ACCCCGUUCUCGAUAGGGACCCUUCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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