Results 1 - 7 of 7 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
17620 | 3' | -51.7 | NC_004466.2 | + | 15110 | 0.71 | 0.593114 |
Target: 5'- aGGGcaGCUCAGAagGCCAcaGGAGGCccccaggGAGCc -3' miRNA: 3'- -CCC--UGAGUCU--UGGU--UCUCCGa------CUUGc -5' |
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17620 | 3' | -51.7 | NC_004466.2 | + | 34964 | 0.67 | 0.812041 |
Target: 5'- cGGACgauaUCAGccGCCAGGAGGUUcuugagaaGAGCGa -3' miRNA: 3'- cCCUG----AGUCu-UGGUUCUCCGA--------CUUGC- -5' |
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17620 | 3' | -51.7 | NC_004466.2 | + | 6195 | 0.66 | 0.857976 |
Target: 5'- -aGGCUCAGAuCCGAcuGGCUGAGa- -3' miRNA: 3'- ccCUGAGUCUuGGUUcuCCGACUUgc -5' |
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17620 | 3' | -51.7 | NC_004466.2 | + | 15821 | 0.66 | 0.86644 |
Target: 5'- cGGGCUCAGcuCC---AGGCUGAAa- -3' miRNA: 3'- cCCUGAGUCuuGGuucUCCGACUUgc -5' |
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17620 | 3' | -51.7 | NC_004466.2 | + | 33063 | 1.11 | 0.00138 |
Target: 5'- gGGGACUCAGAACCAAGAGGCUGAACGu -3' miRNA: 3'- -CCCUGAGUCUUGGUUCUCCGACUUGC- -5' |
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17620 | 3' | -51.7 | NC_004466.2 | + | 17437 | 0.71 | 0.570514 |
Target: 5'- aGGGGCgUUAGGAUCGGuauGGGCUGAGCc -3' miRNA: 3'- -CCCUG-AGUCUUGGUUc--UCCGACUUGc -5' |
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17620 | 3' | -51.7 | NC_004466.2 | + | 30919 | 0.67 | 0.812041 |
Target: 5'- uGGGAUUCucGAuagguuCCAAGAGGUUGucuACu -3' miRNA: 3'- -CCCUGAGu-CUu-----GGUUCUCCGACu--UGc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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