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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
17621 | 3' | -49.2 | NC_004466.2 | + | 41929 | 0.66 | 0.909382 |
Target: 5'- uAAGGUUgUCGUCcUCAgccAGAGCGGgUUCa -3' miRNA: 3'- -UUUCGA-AGCAGcAGU---UCUUGCCgAAG- -5' |
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17621 | 3' | -49.2 | NC_004466.2 | + | 33950 | 0.72 | 0.623505 |
Target: 5'- gAGGGCUUCGaCGUUGAGuccguaGGCUUCg -3' miRNA: 3'- -UUUCGAAGCaGCAGUUCuug---CCGAAG- -5' |
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17621 | 3' | -49.2 | NC_004466.2 | + | 34226 | 1.09 | 0.002913 |
Target: 5'- aAAAGCUUCGUCGUCAAGAACGGCUUCg -3' miRNA: 3'- -UUUCGAAGCAGCAGUUCUUGCCGAAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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