Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 17621 | 5' | -49.4 | NC_004466.2 | + | 23060 | 0.66 | 0.919811 |
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Target: 5'- uGGGACGCCCUaCGAcucCAACAcCGAg -3' miRNA: 3'- uCCUUGUGGGAcGCUua-GUUGUuGUU- -5' |
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| 17621 | 5' | -49.4 | NC_004466.2 | + | 38268 | 0.67 | 0.894309 |
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Target: 5'- gAGGAcccaucaucaAUGCCCUGCGGAUgGcccauauguugguccACAGCAGc -3' miRNA: 3'- -UCCU----------UGUGGGACGCUUAgU---------------UGUUGUU- -5' |
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| 17621 | 5' | -49.4 | NC_004466.2 | + | 36529 | 0.68 | 0.839397 |
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Target: 5'- cAGGAAuuCAUCCUGaacgGGAUUGGCAGCAAc -3' miRNA: 3'- -UCCUU--GUGGGACg---CUUAGUUGUUGUU- -5' |
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| 17621 | 5' | -49.4 | NC_004466.2 | + | 23450 | 0.69 | 0.778558 |
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Target: 5'- cAGGAACACCCUuGUGuauUCGaccuuggccuguGCAGCAu -3' miRNA: 3'- -UCCUUGUGGGA-CGCuu-AGU------------UGUUGUu -5' |
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| 17621 | 5' | -49.4 | NC_004466.2 | + | 39087 | 0.7 | 0.711423 |
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Target: 5'- -cGAGCACCCacUGCGAaauGUCcACGGCAAg -3' miRNA: 3'- ucCUUGUGGG--ACGCU---UAGuUGUUGUU- -5' |
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| 17621 | 5' | -49.4 | NC_004466.2 | + | 34184 | 1.06 | 0.003899 |
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Target: 5'- aAGGAACACCCUGCGAAUCAACAACAAc -3' miRNA: 3'- -UCCUUGUGGGACGCUUAGUUGUUGUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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