Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 17628 | 3' | -56.6 | NC_004466.2 | + | 41557 | 0.66 | 0.566098 |
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Target: 5'- --cUCCCCGgauuGGAUCaugGCCuccCUGGCGUg -3' miRNA: 3'- uucAGGGGU----UCUAGg--UGGu--GACCGCG- -5' |
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| 17628 | 3' | -56.6 | NC_004466.2 | + | 18770 | 0.66 | 0.555105 |
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Target: 5'- ---aCCUCAuGGAUCuCGCCGCUGG-GCu -3' miRNA: 3'- uucaGGGGU-UCUAG-GUGGUGACCgCG- -5' |
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| 17628 | 3' | -56.6 | NC_004466.2 | + | 26137 | 0.68 | 0.410813 |
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Target: 5'- aAGGUCCUCGGGAUggcaCUcCCACUGGCc- -3' miRNA: 3'- -UUCAGGGGUUCUA----GGuGGUGACCGcg -5' |
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| 17628 | 3' | -56.6 | NC_004466.2 | + | 41706 | 0.68 | 0.410813 |
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Target: 5'- gGAG-CCCCGuggugagcucGGAUUCACC-CUGGCaGCc -3' miRNA: 3'- -UUCaGGGGU----------UCUAGGUGGuGACCG-CG- -5' |
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| 17628 | 3' | -56.6 | NC_004466.2 | + | 22018 | 0.71 | 0.28479 |
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Target: 5'- aAAGggCCCCAGGGUaaCgAUCACUGGCGa -3' miRNA: 3'- -UUCa-GGGGUUCUA--GgUGGUGACCGCg -5' |
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| 17628 | 3' | -56.6 | NC_004466.2 | + | 35547 | 0.71 | 0.270498 |
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Target: 5'- -cGUCCCgAAGAUCCA-CAUUGGUcaGCa -3' miRNA: 3'- uuCAGGGgUUCUAGGUgGUGACCG--CG- -5' |
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| 17628 | 3' | -56.6 | NC_004466.2 | + | 39398 | 1.1 | 0.000428 |
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Target: 5'- aAAGUCCCCAAGAUCCACCACUGGCGCa -3' miRNA: 3'- -UUCAGGGGUUCUAGGUGGUGACCGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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