Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 17639 | 3' | -57.2 | NC_004466.2 | + | 20867 | 0.66 | 0.553551 |
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Target: 5'- cCCGaAGGGC-GUUCAGAAGGguguuaaacuuauCCACAa -3' miRNA: 3'- -GGUcUCCCGuCGAGUCUUCC-------------GGUGUc -5' |
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| 17639 | 3' | -57.2 | NC_004466.2 | + | 27177 | 0.66 | 0.543797 |
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Target: 5'- cCCAGAgGGGgAGCUUcGGAGGUCuucgauguaGCGGu -3' miRNA: 3'- -GGUCU-CCCgUCGAGuCUUCCGG---------UGUC- -5' |
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| 17639 | 3' | -57.2 | NC_004466.2 | + | 35222 | 0.67 | 0.470241 |
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Target: 5'- aCgAGGGGGUagcGGCUCAGGuuguGGCUGCc- -3' miRNA: 3'- -GgUCUCCCG---UCGAGUCUu---CCGGUGuc -5' |
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| 17639 | 3' | -57.2 | NC_004466.2 | + | 28152 | 0.67 | 0.460137 |
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Target: 5'- gUAGuuuuuGGGCAGCUUgaAGAAGauGCUACGGg -3' miRNA: 3'- gGUCu----CCCGUCGAG--UCUUC--CGGUGUC- -5' |
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| 17639 | 3' | -57.2 | NC_004466.2 | + | 21420 | 0.69 | 0.366074 |
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Target: 5'- -aAGAaGGCAGCUCuGGAGGCUAaAGa -3' miRNA: 3'- ggUCUcCCGUCGAGuCUUCCGGUgUC- -5' |
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| 17639 | 3' | -57.2 | NC_004466.2 | + | 17760 | 0.69 | 0.357423 |
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Target: 5'- aCGGAGGGCGGUagccUCAGGAGuauugacgcCCAUAGa -3' miRNA: 3'- gGUCUCCCGUCG----AGUCUUCc--------GGUGUC- -5' |
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| 17639 | 3' | -57.2 | NC_004466.2 | + | 2695 | 0.7 | 0.332341 |
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Target: 5'- uCUGGAGugcaaaaaGGCAGCUCAGAuacuugccguuGGGCUGCAu -3' miRNA: 3'- -GGUCUC--------CCGUCGAGUCU-----------UCCGGUGUc -5' |
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| 17639 | 3' | -57.2 | NC_004466.2 | + | 15105 | 1.11 | 0.000325 |
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Target: 5'- gCCAGAGGGCAGCUCAGAAGGCCACAGg -3' miRNA: 3'- -GGUCUCCCGUCGAGUCUUCCGGUGUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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