Results 41 - 46 of 46 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
1764 | 5' | -54.5 | NC_001347.2 | + | 229668 | 0.66 | 0.97457 |
Target: 5'- gGCGGGuGugccGGCGGGg--UGUGGUGGCGg -3' miRNA: 3'- aUGCUC-C----UUGCCUauuGCGCCACCGC- -5' |
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1764 | 5' | -54.5 | NC_001347.2 | + | 155894 | 0.66 | 0.977026 |
Target: 5'- gACGAGucggcgcCGGGUAAauaaaucgGCGGUGGCGu -3' miRNA: 3'- aUGCUCcuu----GCCUAUUg-------CGCCACCGC- -5' |
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1764 | 5' | -54.5 | NC_001347.2 | + | 140538 | 0.66 | 0.981407 |
Target: 5'- gACGGGGGuuuggACGGcaaagGUGACGaCGGgguuccgGGCGg -3' miRNA: 3'- aUGCUCCU-----UGCC-----UAUUGC-GCCa------CCGC- -5' |
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1764 | 5' | -54.5 | NC_001347.2 | + | 189 | 0.66 | 0.97457 |
Target: 5'- gGCGGGuGugccGGCGGGg--UGUGGUGGCGg -3' miRNA: 3'- aUGCUC-C----UUGCCUauuGCGCCACCGC- -5' |
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1764 | 5' | -54.5 | NC_001347.2 | + | 68204 | 0.66 | 0.971654 |
Target: 5'- aGCGAGGAcacgcagACGGcgGAaGCGGUagacgcGGCGc -3' miRNA: 3'- aUGCUCCU-------UGCCuaUUgCGCCA------CCGC- -5' |
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1764 | 5' | -54.5 | NC_001347.2 | + | 188227 | 0.66 | 0.983347 |
Target: 5'- cGCGAGcGGcagaaACGGGUgGACGacgaGGUGGUGc -3' miRNA: 3'- aUGCUC-CU-----UGCCUA-UUGCg---CCACCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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