Results 41 - 46 of 46 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
1764 | 5' | -54.5 | NC_001347.2 | + | 198117 | 0.69 | 0.900662 |
Target: 5'- cGCGuuGA--GGAUAgucGCGCGGUGGCa -3' miRNA: 3'- aUGCucCUugCCUAU---UGCGCCACCGc -5' |
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1764 | 5' | -54.5 | NC_001347.2 | + | 200182 | 0.69 | 0.900035 |
Target: 5'- -gUGAGGAcaagaagACGGA--GCGUGGUGuGCGa -3' miRNA: 3'- auGCUCCU-------UGCCUauUGCGCCAC-CGC- -5' |
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1764 | 5' | -54.5 | NC_001347.2 | + | 225902 | 0.68 | 0.943439 |
Target: 5'- -cUGGGGAACGGGUcGGCGgcCGGUcGGCu -3' miRNA: 3'- auGCUCCUUGCCUA-UUGC--GCCA-CCGc -5' |
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1764 | 5' | -54.5 | NC_001347.2 | + | 228068 | 0.68 | 0.943439 |
Target: 5'- gGCGAGGGGauggguuuauUGGAUAucggugaagcaGCGUGGcGGCGa -3' miRNA: 3'- aUGCUCCUU----------GCCUAU-----------UGCGCCaCCGC- -5' |
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1764 | 5' | -54.5 | NC_001347.2 | + | 228747 | 0.66 | 0.981407 |
Target: 5'- cGCGGGGGACgGGGUAGC-CGaGUGcuGCGc -3' miRNA: 3'- aUGCUCCUUG-CCUAUUGcGC-CAC--CGC- -5' |
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1764 | 5' | -54.5 | NC_001347.2 | + | 229668 | 0.66 | 0.97457 |
Target: 5'- gGCGGGuGugccGGCGGGg--UGUGGUGGCGg -3' miRNA: 3'- aUGCUC-C----UUGCCUauuGCGCCACCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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