Results 41 - 46 of 46 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
1764 | 5' | -54.5 | NC_001347.2 | + | 39870 | 0.69 | 0.91842 |
Target: 5'- -uCGAGGGGCGGGgggguguuuuUAGCGgGGgGGUGa -3' miRNA: 3'- auGCUCCUUGCCU----------AUUGCgCCaCCGC- -5' |
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1764 | 5' | -54.5 | NC_001347.2 | + | 17317 | 0.7 | 0.887703 |
Target: 5'- --aGAGGAGgGGAaGACGCGGUuGCc -3' miRNA: 3'- augCUCCUUgCCUaUUGCGCCAcCGc -5' |
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1764 | 5' | -54.5 | NC_001347.2 | + | 13941 | 0.69 | 0.91842 |
Target: 5'- aACu-GGGGCGGGc-GCGgGGUGGCGa -3' miRNA: 3'- aUGcuCCUUGCCUauUGCgCCACCGC- -5' |
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1764 | 5' | -54.5 | NC_001347.2 | + | 5571 | 0.66 | 0.982203 |
Target: 5'- cGCGguGGGGGCGugccccgcagaccccGGuUAAUGCGGUGGUGg -3' miRNA: 3'- aUGC--UCCUUGC---------------CU-AUUGCGCCACCGC- -5' |
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1764 | 5' | -54.5 | NC_001347.2 | + | 1916 | 0.7 | 0.894293 |
Target: 5'- gGCGcuGGGCGcGGUGcugcccGCGCGGUGGCu -3' miRNA: 3'- aUGCucCUUGC-CUAU------UGCGCCACCGc -5' |
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1764 | 5' | -54.5 | NC_001347.2 | + | 189 | 0.66 | 0.97457 |
Target: 5'- gGCGGGuGugccGGCGGGg--UGUGGUGGCGg -3' miRNA: 3'- aUGCUC-C----UUGCCUauuGCGCCACCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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