Results 41 - 46 of 46 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
1764 | 5' | -54.5 | NC_001347.2 | + | 225902 | 0.68 | 0.943439 |
Target: 5'- -cUGGGGAACGGGUcGGCGgcCGGUcGGCu -3' miRNA: 3'- auGCUCCUUGCCUA-UUGC--GCCA-CCGc -5' |
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1764 | 5' | -54.5 | NC_001347.2 | + | 57297 | 0.68 | 0.951854 |
Target: 5'- -cUGAGGAuaaGCGGGaGAUGUGGaUGGCu -3' miRNA: 3'- auGCUCCU---UGCCUaUUGCGCC-ACCGc -5' |
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1764 | 5' | -54.5 | NC_001347.2 | + | 142561 | 0.67 | 0.955729 |
Target: 5'- gACGAGGcuggcggcgucGGCGGG-AGCaGCGGgGGCGg -3' miRNA: 3'- aUGCUCC-----------UUGCCUaUUG-CGCCaCCGC- -5' |
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1764 | 5' | -54.5 | NC_001347.2 | + | 182404 | 0.67 | 0.959386 |
Target: 5'- gUACGuGGAcGCGG---GCGUGGUGGUu -3' miRNA: 3'- -AUGCuCCU-UGCCuauUGCGCCACCGc -5' |
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1764 | 5' | -54.5 | NC_001347.2 | + | 99180 | 0.67 | 0.962494 |
Target: 5'- gACGA-GAGCGcagccuucauauuGAUAACGCGGuUGGUGu -3' miRNA: 3'- aUGCUcCUUGC-------------CUAUUGCGCC-ACCGC- -5' |
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1764 | 5' | -54.5 | NC_001347.2 | + | 188227 | 0.66 | 0.983347 |
Target: 5'- cGCGAGcGGcagaaACGGGUgGACGacgaGGUGGUGc -3' miRNA: 3'- aUGCUC-CU-----UGCCUA-UUGCg---CCACCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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