Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 17642 | 3' | -59.2 | NC_004466.2 | + | 15807 | 0.67 | 0.367744 |
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Target: 5'- -aCCUAGCuaCCCaaCGGGCUcaGCUCCa -3' miRNA: 3'- uaGGAUUGcgGGGa-GUCCGA--CGAGGa -5' |
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| 17642 | 3' | -59.2 | NC_004466.2 | + | 16508 | 0.67 | 0.350544 |
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Target: 5'- -aCCUGAagcUGCCCCagaccugagcgcUUuGGCUGCUCCg -3' miRNA: 3'- uaGGAUU---GCGGGG------------AGuCCGACGAGGa -5' |
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| 17642 | 3' | -59.2 | NC_004466.2 | + | 12461 | 0.68 | 0.317888 |
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Target: 5'- uUCCUGGCuuaaGCUUCUUAGGggGCUCCa -3' miRNA: 3'- uAGGAUUG----CGGGGAGUCCgaCGAGGa -5' |
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| 17642 | 3' | -59.2 | NC_004466.2 | + | 8072 | 0.72 | 0.169293 |
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Target: 5'- -cCCUGGucggUGCCCCUCAGGCUaaGgUCCa -3' miRNA: 3'- uaGGAUU----GCGGGGAGUCCGA--CgAGGa -5' |
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| 17642 | 3' | -59.2 | NC_004466.2 | + | 14723 | 0.74 | 0.117777 |
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Target: 5'- -cCCUAGCaUCCUUCGGGCUGCUCa- -3' miRNA: 3'- uaGGAUUGcGGGGAGUCCGACGAGga -5' |
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| 17642 | 3' | -59.2 | NC_004466.2 | + | 17378 | 1.06 | 0.000406 |
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Target: 5'- gAUCCUAACGCCCCUCAGGCUGCUCCUa -3' miRNA: 3'- -UAGGAUUGCGGGGAGUCCGACGAGGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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