Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
17646 | 3' | -54.9 | NC_004466.2 | + | 6631 | 0.67 | 0.618309 |
Target: 5'- aUCGCCUC-CCgAGuCUCACUAUcuuugGGgGCc -3' miRNA: 3'- -AGUGGAGaGG-UCuGAGUGAUG-----CCgCG- -5' |
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17646 | 3' | -54.9 | NC_004466.2 | + | 32318 | 0.67 | 0.607165 |
Target: 5'- cUACCUCcuucgUCCAGGCcCGgaGCGGUGUu -3' miRNA: 3'- aGUGGAG-----AGGUCUGaGUgaUGCCGCG- -5' |
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17646 | 3' | -54.9 | NC_004466.2 | + | 29601 | 0.69 | 0.530271 |
Target: 5'- gUCGCCUC-CC-GACUCACU-UGGCc- -3' miRNA: 3'- -AGUGGAGaGGuCUGAGUGAuGCCGcg -5' |
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17646 | 3' | -54.9 | NC_004466.2 | + | 26300 | 1.13 | 0.000447 |
Target: 5'- aUCACCUCUCCAGACUCACUACGGCGCa -3' miRNA: 3'- -AGUGGAGAGGUCUGAGUGAUGCCGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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