Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 17649 | 5' | -50.9 | NC_004466.2 | + | 44855 | 0.66 | 0.885853 |
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Target: 5'- aUUCCCGUUcgucuucGCCaaggacaagGCCACuCGUCUggAGCg -3' miRNA: 3'- -AGGGGCAA-------UGG---------CGGUG-GUAGAaaUUG- -5' |
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| 17649 | 5' | -50.9 | NC_004466.2 | + | 38333 | 0.66 | 0.853452 |
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Target: 5'- aUCCUCGgacGCCGCCaggauagcGCCAUCggucauccgaUUGACg -3' miRNA: 3'- -AGGGGCaa-UGGCGG--------UGGUAGa---------AAUUG- -5' |
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| 17649 | 5' | -50.9 | NC_004466.2 | + | 38113 | 0.69 | 0.743358 |
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Target: 5'- aUCCCCGUgacUGCUGgCCAgUAUCgugAGCa -3' miRNA: 3'- -AGGGGCA---AUGGC-GGUgGUAGaaaUUG- -5' |
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| 17649 | 5' | -50.9 | NC_004466.2 | + | 33037 | 0.69 | 0.721234 |
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Target: 5'- gUCUCCGUcGCCuuGCCcCCAUCUUccugAACa -3' miRNA: 3'- -AGGGGCAaUGG--CGGuGGUAGAAa---UUG- -5' |
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| 17649 | 5' | -50.9 | NC_004466.2 | + | 34714 | 0.7 | 0.66445 |
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Target: 5'- uUCCCauaugGUUGCaGCCACCAUCgccaAGCa -3' miRNA: 3'- -AGGGg----CAAUGgCGGUGGUAGaaa-UUG- -5' |
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| 17649 | 5' | -50.9 | NC_004466.2 | + | 29130 | 1.12 | 0.00137 |
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Target: 5'- aUCCCCGUUACCGCCACCAUCUUUAACa -3' miRNA: 3'- -AGGGGCAAUGGCGGUGGUAGAAAUUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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