Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
17651 | 3' | -54.1 | NC_004466.2 | + | 32714 | 1.09 | 0.000748 |
Target: 5'- uGCCUGCUGCGCAUCAACUGCUGGAUAg -3' miRNA: 3'- -CGGACGACGCGUAGUUGACGACCUAU- -5' |
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17651 | 3' | -54.1 | NC_004466.2 | + | 23423 | 0.73 | 0.292802 |
Target: 5'- gGCCUG-UGCaGCAUCAGCUuCUGGAa- -3' miRNA: 3'- -CGGACgACG-CGUAGUUGAcGACCUau -5' |
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17651 | 3' | -54.1 | NC_004466.2 | + | 34762 | 0.68 | 0.553792 |
Target: 5'- uGCCUG--GgGUAUCGAuCUGCUGGAg- -3' miRNA: 3'- -CGGACgaCgCGUAGUU-GACGACCUau -5' |
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17651 | 3' | -54.1 | NC_004466.2 | + | 38017 | 0.66 | 0.679813 |
Target: 5'- gGCCUGggGCGgAUUGAUaGCUGGGg- -3' miRNA: 3'- -CGGACgaCGCgUAGUUGaCGACCUau -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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