Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 17659 | 3' | -59.8 | NC_004466.2 | + | 16948 | 0.66 | 0.421367 |
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Target: 5'- aGGaGCUuGACCCCgagGGAGUaacCCCUGGa -3' miRNA: 3'- cCC-CGAcUUGGGGag-CCUCA---GGGACU- -5' |
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| 17659 | 3' | -59.8 | NC_004466.2 | + | 727 | 0.67 | 0.368025 |
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Target: 5'- aGGGGCuUGAcaaACCCCgCGGAa--CCUGGc -3' miRNA: 3'- -CCCCG-ACU---UGGGGaGCCUcagGGACU- -5' |
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| 17659 | 3' | -59.8 | NC_004466.2 | + | 5936 | 0.68 | 0.343148 |
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Target: 5'- cGGGGgUGGuggUCUCGGGGUCCUUGu -3' miRNA: 3'- -CCCCgACUuggGGAGCCUCAGGGACu -5' |
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| 17659 | 3' | -59.8 | NC_004466.2 | + | 12591 | 0.68 | 0.343148 |
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Target: 5'- aGGGGUgaauagGAuaaaAUCCCUgGGAGaUCCUUGGg -3' miRNA: 3'- -CCCCGa-----CU----UGGGGAgCCUC-AGGGACU- -5' |
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| 17659 | 3' | -59.8 | NC_004466.2 | + | 35646 | 0.72 | 0.192864 |
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Target: 5'- aGGGGUUGAca-CCUCuGAGUUCCUGGa -3' miRNA: 3'- -CCCCGACUuggGGAGcCUCAGGGACU- -5' |
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| 17659 | 3' | -59.8 | NC_004466.2 | + | 40843 | 1.1 | 0.000262 |
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Target: 5'- uGGGGCUGAACCCCUCGGAGUCCCUGAu -3' miRNA: 3'- -CCCCGACUUGGGGAGCCUCAGGGACU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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