miRNA: 3'- -GCUaGAcuGGG-GGUGGAGUAGGGcgu -5'
miRNA: 3'- gCUAGAC--UGGGGguGGAGUAGGG-CGU- -5'
miRNA: 3'- -GCUAGACUGGGGGUGGAGUAGGGCGU- -5'
Results 1 - 3 of 3 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 17660 | 3' | -58.3 | NC_004466.2 | + | 20669 | 0.66 | 0.484397 |
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Target: 5'- cCGG-CUucCCCuCCACCUCGUCCCa-- -3' miRNA: 3'- -GCUaGAcuGGG-GGUGGAGUAGGGcgu -5' |
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| 17660 | 3' | -58.3 | NC_004466.2 | + | 42487 | 0.7 | 0.306405 |
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Target: 5'- gGGUUUGcaGCCCCguCCUCGUCCUgGCGg -3' miRNA: 3'- gCUAGAC--UGGGGguGGAGUAGGG-CGU- -5' |
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| 17660 | 3' | -58.3 | NC_004466.2 | + | 41360 | 1.08 | 0.000447 |
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Target: 5'- aCGAUCUGACCCCCACCUCAUCCCGCAc -3' miRNA: 3'- -GCUAGACUGGGGGUGGAGUAGGGCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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