Results 1 - 6 of 6 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
17661 | 3' | -50.8 | NC_004466.2 | + | 4061 | 0.66 | 0.860801 |
Target: 5'- gGACCuggGGGGUUGCUGAAgGguUCa -3' miRNA: 3'- aCUGGua-CUCCAGCGGCUUgUauAGa -5' |
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17661 | 3' | -50.8 | NC_004466.2 | + | 6797 | 0.72 | 0.570428 |
Target: 5'- gGGCCAgGAGGUCaucaCCGAGCAaAUCa -3' miRNA: 3'- aCUGGUaCUCCAGc---GGCUUGUaUAGa -5' |
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17661 | 3' | -50.8 | NC_004466.2 | + | 8316 | 0.66 | 0.860801 |
Target: 5'- cGACUccGAGG-CGUucaaGAGCAUAUCUu -3' miRNA: 3'- aCUGGuaCUCCaGCGg---CUUGUAUAGA- -5' |
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17661 | 3' | -50.8 | NC_004466.2 | + | 22177 | 0.66 | 0.885427 |
Target: 5'- cGGCCuuacgGuGGUucCGCCGAGCAUcgCc -3' miRNA: 3'- aCUGGua---CuCCA--GCGGCUUGUAuaGa -5' |
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17661 | 3' | -50.8 | NC_004466.2 | + | 40774 | 0.71 | 0.615185 |
Target: 5'- aGACCAUGAGGgccUCGCCGu-CGUuguacugGUCa -3' miRNA: 3'- aCUGGUACUCC---AGCGGCuuGUA-------UAGa -5' |
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17661 | 3' | -50.8 | NC_004466.2 | + | 42617 | 1.08 | 0.002538 |
Target: 5'- aUGACCAUGAGGUCGCCGAACAUAUCUu -3' miRNA: 3'- -ACUGGUACUCCAGCGGCUUGUAUAGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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