miRNA: 3'- -GGAACUG----------UCGGAGAcCCAGCCA---UCGc -5'
miRNA: 3'- -GGaACU--GUCGGAGAC-CCaGCCA--UCGC- -5'
miRNA: 3'- -GGAACUGUCGGAGACCCAGCCAUCGC- -5'
Results 1 - 3 of 3 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 17662 | 5' | -55.8 | NC_004466.2 | + | 10669 | 0.67 | 0.564302 |
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Target: 5'- uCCUUGACcagcagagccAGUCUCUaGGUUGGUauuGGCa -3' miRNA: 3'- -GGAACUG----------UCGGAGAcCCAGCCA---UCGc -5' |
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| 17662 | 5' | -55.8 | NC_004466.2 | + | 15204 | 0.69 | 0.44941 |
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Target: 5'- cCCcUGAggUGGCCUCUGaGGcCGGUgcAGCGg -3' miRNA: 3'- -GGaACU--GUCGGAGAC-CCaGCCA--UCGC- -5' |
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| 17662 | 5' | -55.8 | NC_004466.2 | + | 42968 | 1.1 | 0.000686 |
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Target: 5'- uCCUUGACAGCCUCUGGGUCGGUAGCGc -3' miRNA: 3'- -GGAACUGUCGGAGACCCAGCCAUCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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