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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1772 | 3' | -54.9 | NC_001347.2 | + | 136729 | 0.66 | 0.967876 |
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Target: 5'- gGGGGGGGUGagGGgGGGggGUUACGg- -3' miRNA: 3'- -UCUCUCCACa-UCgCCCgaCAGUGUag -5' |
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| 1772 | 3' | -54.9 | NC_001347.2 | + | 35530 | 0.68 | 0.926431 |
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Target: 5'- uGGGAGGUGgc-UGGGC--UCACGUCa -3' miRNA: 3'- uCUCUCCACaucGCCCGacAGUGUAG- -5' |
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| 1772 | 3' | -54.9 | NC_001347.2 | + | 182201 | 0.68 | 0.909494 |
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Target: 5'- cGGGGugcGG-GUGGCGGGCUgcGUCGCcuUCg -3' miRNA: 3'- -UCUCu--CCaCAUCGCCCGA--CAGUGu-AG- -5' |
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| 1772 | 3' | -54.9 | NC_001347.2 | + | 15005 | 0.71 | 0.804642 |
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Target: 5'- uGGAGAGccgucguugGUGGUGGGCUGUC-CGUg -3' miRNA: 3'- -UCUCUCca-------CAUCGCCCGACAGuGUAg -5' |
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| 1772 | 3' | -54.9 | NC_001347.2 | + | 198915 | 0.72 | 0.749403 |
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Target: 5'- --cGAGGUGUAGUGGGCgGUauagaagagCACGUUg -3' miRNA: 3'- ucuCUCCACAUCGCCCGaCA---------GUGUAG- -5' |
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| 1772 | 3' | -54.9 | NC_001347.2 | + | 228893 | 0.73 | 0.710434 |
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Target: 5'- -uAGAGGUGuUGGCGGGCcUGUgCGCAa- -3' miRNA: 3'- ucUCUCCAC-AUCGCCCG-ACA-GUGUag -5' |
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| 1772 | 3' | -54.9 | NC_001347.2 | + | 34101 | 1.1 | 0.004104 |
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Target: 5'- cAGAGAGGUGUAGCGGGCUGUCACAUCa -3' miRNA: 3'- -UCUCUCCACAUCGCCCGACAGUGUAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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