Results 1 - 16 of 16 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
17746 | 3' | -46.2 | NC_004629.1 | + | 170230 | 0.66 | 0.999999 |
Target: 5'- gAUGGAUCAccGUUAACAgaaaUGUCAa-- -3' miRNA: 3'- aUACCUAGUuaCGGUUGU----ACAGUagu -5' |
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17746 | 3' | -46.2 | NC_004629.1 | + | 114333 | 0.67 | 0.999995 |
Target: 5'- gAUGGAUCAAc-CCAuaGCAUGUCGg-- -3' miRNA: 3'- aUACCUAGUUacGGU--UGUACAGUagu -5' |
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17746 | 3' | -46.2 | NC_004629.1 | + | 196479 | 0.67 | 0.999995 |
Target: 5'- aUAUGGAUUAAUuggGCU---GUGUCGUCAu -3' miRNA: 3'- -AUACCUAGUUA---CGGuugUACAGUAGU- -5' |
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17746 | 3' | -46.2 | NC_004629.1 | + | 79671 | 0.68 | 0.999949 |
Target: 5'- cAUGGAUCuggucuauuuaGAUaCCAgaaauGCAUGUCAUCAc -3' miRNA: 3'- aUACCUAG-----------UUAcGGU-----UGUACAGUAGU- -5' |
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17746 | 3' | -46.2 | NC_004629.1 | + | 66004 | 0.69 | 0.999911 |
Target: 5'- aGUGGAUCuAAUGgCAGCA-GUCAaCAa -3' miRNA: 3'- aUACCUAG-UUACgGUUGUaCAGUaGU- -5' |
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17746 | 3' | -46.2 | NC_004629.1 | + | 76360 | 0.69 | 0.999895 |
Target: 5'- -nUGGAccacguaguucaaucUCAGUGCCAACuaguUCAUCAa -3' miRNA: 3'- auACCU---------------AGUUACGGUUGuac-AGUAGU- -5' |
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17746 | 3' | -46.2 | NC_004629.1 | + | 229697 | 0.69 | 0.999849 |
Target: 5'- uUAUGGAUUggUGUgAGCggGUUAUUAa -3' miRNA: 3'- -AUACCUAGuuACGgUUGuaCAGUAGU- -5' |
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17746 | 3' | -46.2 | NC_004629.1 | + | 47874 | 0.69 | 0.999849 |
Target: 5'- gUAUGGGUCuuuAUGCCAAaguUGUUgAUCAa -3' miRNA: 3'- -AUACCUAGu--UACGGUUgu-ACAG-UAGU- -5' |
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17746 | 3' | -46.2 | NC_004629.1 | + | 192559 | 0.69 | 0.999805 |
Target: 5'- cGUGGuu--AUGCCAAUAUGUCAa-- -3' miRNA: 3'- aUACCuaguUACGGUUGUACAGUagu -5' |
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17746 | 3' | -46.2 | NC_004629.1 | + | 183419 | 0.69 | 0.999751 |
Target: 5'- gGUGGGUCAGUGCaAAC-UGUCGa-- -3' miRNA: 3'- aUACCUAGUUACGgUUGuACAGUagu -5' |
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17746 | 3' | -46.2 | NC_004629.1 | + | 28370 | 0.7 | 0.999229 |
Target: 5'- --aGGAUCAGcaucUGCUAaaccuucuaGCAUGUCGUCu -3' miRNA: 3'- auaCCUAGUU----ACGGU---------UGUACAGUAGu -5' |
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17746 | 3' | -46.2 | NC_004629.1 | + | 268940 | 0.71 | 0.998842 |
Target: 5'- -uUGGAUCAAUGCCcaguCGUuUCAUCc -3' miRNA: 3'- auACCUAGUUACGGuu--GUAcAGUAGu -5' |
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17746 | 3' | -46.2 | NC_004629.1 | + | 126580 | 0.71 | 0.998842 |
Target: 5'- gAUGGAauaAAUGCUGAUggGUCAUCAa -3' miRNA: 3'- aUACCUag-UUACGGUUGuaCAGUAGU- -5' |
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17746 | 3' | -46.2 | NC_004629.1 | + | 76387 | 0.71 | 0.9983 |
Target: 5'- -cUGGAUCAccaccgGCCuuACAUGUCAUUg -3' miRNA: 3'- auACCUAGUua----CGGu-UGUACAGUAGu -5' |
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17746 | 3' | -46.2 | NC_004629.1 | + | 164672 | 0.75 | 0.976893 |
Target: 5'- -uUGGGUCAAUGCCucuUAUGUCAg-- -3' miRNA: 3'- auACCUAGUUACGGuu-GUACAGUagu -5' |
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17746 | 3' | -46.2 | NC_004629.1 | + | 6062 | 1.09 | 0.040879 |
Target: 5'- uUAUGGAUCAAUGCCAACAUGUCAUCAa -3' miRNA: 3'- -AUACCUAGUUACGGUUGUACAGUAGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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