Results 1 - 12 of 12 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
17749 | 3' | -45.6 | NC_004629.1 | + | 88955 | 0.66 | 1 |
Target: 5'- aGAuc-GAUAGaaaGCUCACAAUGCUAUu -3' miRNA: 3'- aCUuuuCUAUUa--CGGGUGUUACGGUG- -5' |
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17749 | 3' | -45.6 | NC_004629.1 | + | 72071 | 0.66 | 0.999999 |
Target: 5'- -cGGAAGAUAGUGgCCACAuc-CUACa -3' miRNA: 3'- acUUUUCUAUUACgGGUGUuacGGUG- -5' |
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17749 | 3' | -45.6 | NC_004629.1 | + | 211409 | 0.66 | 0.999999 |
Target: 5'- gGGAAGGGUuuuaGGUGCUaaaCGCAuucaGUGCCAUg -3' miRNA: 3'- aCUUUUCUA----UUACGG---GUGU----UACGGUG- -5' |
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17749 | 3' | -45.6 | NC_004629.1 | + | 101439 | 0.67 | 0.999998 |
Target: 5'- ----cAGGUAAUGUUCaaACAGUGCCAa -3' miRNA: 3'- acuuuUCUAUUACGGG--UGUUACGGUg -5' |
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17749 | 3' | -45.6 | NC_004629.1 | + | 237010 | 0.68 | 0.999991 |
Target: 5'- -aAAAAGAUGAUaaCCaaauGCGAUGCCACc -3' miRNA: 3'- acUUUUCUAUUAcgGG----UGUUACGGUG- -5' |
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17749 | 3' | -45.6 | NC_004629.1 | + | 194717 | 0.68 | 0.999982 |
Target: 5'- cUGGGAAuGGUGGUGUaugggagaauauCC-CAAUGCCGCa -3' miRNA: 3'- -ACUUUU-CUAUUACG------------GGuGUUACGGUG- -5' |
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17749 | 3' | -45.6 | NC_004629.1 | + | 267029 | 0.68 | 0.999982 |
Target: 5'- aGAAuAAGAgca--CCCACGGUGCCAa -3' miRNA: 3'- aCUU-UUCUauuacGGGUGUUACGGUg -5' |
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17749 | 3' | -45.6 | NC_004629.1 | + | 160312 | 0.69 | 0.999941 |
Target: 5'- aGAAGAGG-AGUGCUCAUAuugGaCCACa -3' miRNA: 3'- aCUUUUCUaUUACGGGUGUua-C-GGUG- -5' |
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17749 | 3' | -45.6 | NC_004629.1 | + | 172249 | 0.69 | 0.999919 |
Target: 5'- uUGucu-GAUAAaaacaacUGCCCACAcugGCCACg -3' miRNA: 3'- -ACuuuuCUAUU-------ACGGGUGUua-CGGUG- -5' |
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17749 | 3' | -45.6 | NC_004629.1 | + | 195464 | 0.69 | 0.999897 |
Target: 5'- uUGGGAAGAUAAUGUCUGguAUGgCAUc -3' miRNA: 3'- -ACUUUUCUAUUACGGGUguUACgGUG- -5' |
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17749 | 3' | -45.6 | NC_004629.1 | + | 169474 | 0.72 | 0.998936 |
Target: 5'- -aAAAGGaAUGAUGUCCA--GUGCCACg -3' miRNA: 3'- acUUUUC-UAUUACGGGUguUACGGUG- -5' |
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17749 | 3' | -45.6 | NC_004629.1 | + | 25884 | 1.11 | 0.036313 |
Target: 5'- uUGAAAAGAUAAUGCCCACAAUGCCACc -3' miRNA: 3'- -ACUUUUCUAUUACGGGUGUUACGGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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