Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 17749 | 5' | -48.3 | NC_004629.1 | + | 202705 | 0.66 | 0.999988 |
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Target: 5'- gUCAUGGCGAUA-UUGGACuACUUg--- -3' miRNA: 3'- -AGUACCGCUGUgAAUCUG-UGGAaagg -5' |
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| 17749 | 5' | -48.3 | NC_004629.1 | + | 180240 | 0.66 | 0.999981 |
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Target: 5'- aCAUGGCGAUcUUUAaguucaguauuaauGACACCa-UCCa -3' miRNA: 3'- aGUACCGCUGuGAAU--------------CUGUGGaaAGG- -5' |
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| 17749 | 5' | -48.3 | NC_004629.1 | + | 105787 | 0.66 | 0.999977 |
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Target: 5'- -gAUGGUGAUACUUAuACaACCaUUCUg -3' miRNA: 3'- agUACCGCUGUGAAUcUG-UGGaAAGG- -5' |
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| 17749 | 5' | -48.3 | NC_004629.1 | + | 51602 | 0.66 | 0.999969 |
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Target: 5'- cUCAUGGUGugGaccuaUUGGACAgUUgcgCCg -3' miRNA: 3'- -AGUACCGCugUg----AAUCUGUgGAaa-GG- -5' |
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| 17749 | 5' | -48.3 | NC_004629.1 | + | 166657 | 0.66 | 0.999969 |
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Target: 5'- -aGUGGUGcCAgUUAGAUaaGCCUUaCCa -3' miRNA: 3'- agUACCGCuGUgAAUCUG--UGGAAaGG- -5' |
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| 17749 | 5' | -48.3 | NC_004629.1 | + | 42389 | 0.67 | 0.999879 |
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Target: 5'- aUCAuuUGGUGACGCUgguGAucCACCUUaUUCa -3' miRNA: 3'- -AGU--ACCGCUGUGAau-CU--GUGGAA-AGG- -5' |
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| 17749 | 5' | -48.3 | NC_004629.1 | + | 192629 | 0.7 | 0.997472 |
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Target: 5'- -aAUGGCGGguuuaggauauaaauUGCUUGGAuCACCUUUCa -3' miRNA: 3'- agUACCGCU---------------GUGAAUCU-GUGGAAAGg -5' |
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| 17749 | 5' | -48.3 | NC_004629.1 | + | 106101 | 0.71 | 0.995637 |
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Target: 5'- cCAUGGCGAUGCU--GAUAUCUUaacgCCa -3' miRNA: 3'- aGUACCGCUGUGAauCUGUGGAAa---GG- -5' |
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| 17749 | 5' | -48.3 | NC_004629.1 | + | 25849 | 0.98 | 0.120692 |
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Target: 5'- uUCAU-GCGACACUUAGACACCUUUCCc -3' miRNA: 3'- -AGUAcCGCUGUGAAUCUGUGGAAAGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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