Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 17751 | 3' | -46.3 | NC_004629.1 | + | 244029 | 0.66 | 0.999999 |
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Target: 5'- -cCUCUCCUu-----UGGGAGAGGGUu -3' miRNA: 3'- uaGAGAGGAuuugcuAUCUUCUCUCG- -5' |
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| 17751 | 3' | -46.3 | NC_004629.1 | + | 158056 | 0.66 | 0.999997 |
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Target: 5'- -cCUCUCCcAAA----AGGAGAGGGCa -3' miRNA: 3'- uaGAGAGGaUUUgcuaUCUUCUCUCG- -5' |
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| 17751 | 3' | -46.3 | NC_004629.1 | + | 114102 | 0.68 | 0.999986 |
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Target: 5'- -cCUCUCCUu--CG---GGAGAGGGCa -3' miRNA: 3'- uaGAGAGGAuuuGCuauCUUCUCUCG- -5' |
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| 17751 | 3' | -46.3 | NC_004629.1 | + | 231327 | 0.68 | 0.999951 |
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Target: 5'- ---cCUCCUAGACGAUAuacGGGAGUc -3' miRNA: 3'- uagaGAGGAUUUGCUAUcuuCUCUCG- -5' |
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| 17751 | 3' | -46.3 | NC_004629.1 | + | 158354 | 0.71 | 0.999275 |
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Target: 5'- -cCUCUCCUuu----UGGGAGAGGGCu -3' miRNA: 3'- uaGAGAGGAuuugcuAUCUUCUCUCG- -5' |
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| 17751 | 3' | -46.3 | NC_004629.1 | + | 19772 | 0.72 | 0.998404 |
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Target: 5'- --aUCaCCUAAGCGAUGGAGGAaguccGGGCc -3' miRNA: 3'- uagAGaGGAUUUGCUAUCUUCU-----CUCG- -5' |
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| 17751 | 3' | -46.3 | NC_004629.1 | + | 52894 | 1.11 | 0.033978 |
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Target: 5'- gAUCUCUCCUAAACGAUAGAAGAGAGCc -3' miRNA: 3'- -UAGAGAGGAUUUGCUAUCUUCUCUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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