miRNA: 3'- uGAGGAUUguaUUCUgCCCUGAGaAG- -5'
miRNA: 3'- -UGAGGAU---UGUAuu----CUGCCCUGAGAaG- -5'
miRNA: 3'- -UGagGAUUGuauUCUGcCCUGAGAAG- -5'
miRNA: 3'- -UGaGGa--UUGUaUUCUGCCCUGAGAAG- -5'
miRNA: 3'- -UGAGGAUUGUAUUCUGCCCUGAGAAG- -5'
Results 1 - 5 of 5 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 17752 | 3' | -48.4 | NC_004629.1 | + | 114569 | 0.66 | 0.999966 |
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Target: 5'- uCUCCUAAag-GAGA-GGGACUUaUCu -3' miRNA: 3'- uGAGGAUUguaUUCUgCCCUGAGaAG- -5' |
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| 17752 | 3' | -48.4 | NC_004629.1 | + | 67181 | 0.66 | 0.999921 |
|
Target: 5'- aGCUUCUAcagGCAUuugcuuGGCGGGGCUUUcCa -3' miRNA: 3'- -UGAGGAU---UGUAuu----CUGCCCUGAGAaG- -5' |
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| 17752 | 3' | -48.4 | NC_004629.1 | + | 225588 | 0.67 | 0.999867 |
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Target: 5'- aGCaaCUAGCuu-AGGCuGGGCUCUUCg -3' miRNA: 3'- -UGagGAUUGuauUCUGcCCUGAGAAG- -5' |
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| 17752 | 3' | -48.4 | NC_004629.1 | + | 6090 | 0.74 | 0.963407 |
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Target: 5'- gAC-CCaggAAUAaAAGAUGGGACUCUUCg -3' miRNA: 3'- -UGaGGa--UUGUaUUCUGCCCUGAGAAG- -5' |
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| 17752 | 3' | -48.4 | NC_004629.1 | + | 97844 | 1.09 | 0.025576 |
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Target: 5'- aACUCCUAACAUAAGACGGGACUCUUCc -3' miRNA: 3'- -UGAGGAUUGUAUUCUGCCCUGAGAAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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