Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 17753 | 3' | -48.4 | NC_004629.1 | + | 195102 | 0.66 | 0.999919 |
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Target: 5'- -uUAUCUAGCGcacuUCAUGGGuccAGCUggUGCa -3' miRNA: 3'- auAUAGGUCGCu---AGUAUCC---UUGG--ACG- -5' |
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| 17753 | 3' | -48.4 | NC_004629.1 | + | 119918 | 0.67 | 0.999777 |
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Target: 5'- gGUGUCCAuCGGUagaGUuacAGGAGCgUGCa -3' miRNA: 3'- aUAUAGGUcGCUAg--UA---UCCUUGgACG- -5' |
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| 17753 | 3' | -48.4 | NC_004629.1 | + | 135887 | 0.69 | 0.998501 |
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Target: 5'- --aAUCCAGCauaagCAUGGaAACCUGCc -3' miRNA: 3'- auaUAGGUCGcua--GUAUCcUUGGACG- -5' |
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| 17753 | 3' | -48.4 | NC_004629.1 | + | 60567 | 0.69 | 0.997849 |
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Target: 5'- aGUAUCgCGGCGAUUugaauauuGGAaaguACCUGCu -3' miRNA: 3'- aUAUAG-GUCGCUAGuau-----CCU----UGGACG- -5' |
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| 17753 | 3' | -48.4 | NC_004629.1 | + | 20679 | 0.7 | 0.996441 |
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Target: 5'- gAUGUCCGGacgGAUC--AGGGGCCgGCg -3' miRNA: 3'- aUAUAGGUCg--CUAGuaUCCUUGGaCG- -5' |
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| 17753 | 3' | -48.4 | NC_004629.1 | + | 20355 | 0.7 | 0.995829 |
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Target: 5'- gGUAUCCGG-GAUCGUAGccGCCgGCc -3' miRNA: 3'- aUAUAGGUCgCUAGUAUCcuUGGaCG- -5' |
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| 17753 | 3' | -48.4 | NC_004629.1 | + | 2276 | 0.71 | 0.990122 |
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Target: 5'- aUAUGUUCAGUGGUgGUA-GAACCUGg -3' miRNA: 3'- -AUAUAGGUCGCUAgUAUcCUUGGACg -5' |
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| 17753 | 3' | -48.4 | NC_004629.1 | + | 104724 | 1.12 | 0.016587 |
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Target: 5'- cUAUAUCCAGCGAUCAUAGGAACCUGCa -3' miRNA: 3'- -AUAUAGGUCGCUAGUAUCCUUGGACG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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