Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 17754 | 3' | -46.9 | NC_004629.1 | + | 114877 | 0.66 | 0.999994 |
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Target: 5'- aAUUCA--GAUAAGUCCCUCUccuuuaggAGAGg -3' miRNA: 3'- -UAAGUauUUAUUCGGGGAGGca------UCUC- -5' |
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| 17754 | 3' | -46.9 | NC_004629.1 | + | 10590 | 0.67 | 0.999984 |
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Target: 5'- --aUAUAA---GGCCCCUCCcuucggGUAGGGg -3' miRNA: 3'- uaaGUAUUuauUCGGGGAGG------CAUCUC- -5' |
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| 17754 | 3' | -46.9 | NC_004629.1 | + | 125916 | 0.67 | 0.999979 |
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Target: 5'- --gCAUAA--AAGCCCCUCUacGGAGg -3' miRNA: 3'- uaaGUAUUuaUUCGGGGAGGcaUCUC- -5' |
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| 17754 | 3' | -46.9 | NC_004629.1 | + | 71311 | 0.67 | 0.999961 |
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Target: 5'- --aCGUAAGUAAGacgacauaaaCCCCUCUccauGUGGAGa -3' miRNA: 3'- uaaGUAUUUAUUC----------GGGGAGG----CAUCUC- -5' |
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| 17754 | 3' | -46.9 | NC_004629.1 | + | 266663 | 0.69 | 0.999681 |
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Target: 5'- -aUCAUAuu---GCCCCUCCauaUGGAGg -3' miRNA: 3'- uaAGUAUuuauuCGGGGAGGc--AUCUC- -5' |
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| 17754 | 3' | -46.9 | NC_004629.1 | + | 266343 | 0.69 | 0.999498 |
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Target: 5'- ---aAUGAcaaacAUAAGCCCCUCCauaUGGAGg -3' miRNA: 3'- uaagUAUU-----UAUUCGGGGAGGc--AUCUC- -5' |
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| 17754 | 3' | -46.9 | NC_004629.1 | + | 274107 | 0.72 | 0.996019 |
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Target: 5'- -aUCGUAGAUuAGUCCUUCCaGUGGAu -3' miRNA: 3'- uaAGUAUUUAuUCGGGGAGG-CAUCUc -5' |
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| 17754 | 3' | -46.9 | NC_004629.1 | + | 126234 | 1.09 | 0.035388 |
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Target: 5'- cAUUCAUAAAUAAGCCCCUCCGUAGAGg -3' miRNA: 3'- -UAAGUAUUUAUUCGGGGAGGCAUCUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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