Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 17755 | 5' | -45.2 | NC_004629.1 | + | 10885 | 0.67 | 0.999999 |
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Target: 5'- aGGGAGGGGccUAUaUCGUUACUUGc- -3' miRNA: 3'- -CUCUCCCCaaAUAcGGUAAUGAAUua -5' |
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| 17755 | 5' | -45.2 | NC_004629.1 | + | 207989 | 0.67 | 0.999998 |
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Target: 5'- cGGGAGGGc-UUAUGCCGUUaaACUUu-- -3' miRNA: 3'- -CUCUCCCcaAAUACGGUAA--UGAAuua -5' |
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| 17755 | 5' | -45.2 | NC_004629.1 | + | 35170 | 0.68 | 0.999986 |
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Target: 5'- cGGGAGGGc-UUAUGCCAUUAagaUAAUa -3' miRNA: 3'- -CUCUCCCcaAAUACGGUAAUga-AUUA- -5' |
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| 17755 | 5' | -45.2 | NC_004629.1 | + | 126212 | 0.69 | 0.999974 |
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Target: 5'- uAGAGGGGcuUUUAUGCU--UAUUUGAUa -3' miRNA: 3'- cUCUCCCC--AAAUACGGuaAUGAAUUA- -5' |
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| 17755 | 5' | -45.2 | NC_004629.1 | + | 143068 | 0.69 | 0.999974 |
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Target: 5'- uGGAGGGGaUUUAUGCUGUUAa----- -3' miRNA: 3'- cUCUCCCC-AAAUACGGUAAUgaauua -5' |
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| 17755 | 5' | -45.2 | NC_004629.1 | + | 70491 | 0.69 | 0.999965 |
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Target: 5'- aGGGAGGGaucaUUUAUGCCAuUUACUa--- -3' miRNA: 3'- -CUCUCCCc---AAAUACGGU-AAUGAauua -5' |
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| 17755 | 5' | -45.2 | NC_004629.1 | + | 44014 | 0.7 | 0.999916 |
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Target: 5'- uGGAGGGGguuaUAUGCCGUUuuaUUAGa -3' miRNA: 3'- cUCUCCCCaa--AUACGGUAAug-AAUUa -5' |
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| 17755 | 5' | -45.2 | NC_004629.1 | + | 265465 | 0.84 | 0.737283 |
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Target: 5'- gGAGAGGGGUUUAUGCCGUaauuaUACa---- -3' miRNA: 3'- -CUCUCCCCAAAUACGGUA-----AUGaauua -5' |
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| 17755 | 5' | -45.2 | NC_004629.1 | + | 71620 | 0.88 | 0.565374 |
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Target: 5'- gGAGAGGGGUUUAUGUCGUcuUACUUAc- -3' miRNA: 3'- -CUCUCCCCAAAUACGGUA--AUGAAUua -5' |
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| 17755 | 5' | -45.2 | NC_004629.1 | + | 126936 | 1.06 | 0.07128 |
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Target: 5'- gGAGAGGGGUUUAUGCCAUUACUUAAUu -3' miRNA: 3'- -CUCUCCCCAAAUACGGUAAUGAAUUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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