Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 17756 | 5' | -49.4 | NC_004629.1 | + | 124571 | 0.68 | 0.999233 |
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Target: 5'- uUGUUGauauccacccUGCAucaugguguUACCACCCAUaCGCAUCu -3' miRNA: 3'- cGCAAU----------GCGU---------AUGGUGGGUA-GUGUAG- -5' |
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| 17756 | 5' | -49.4 | NC_004629.1 | + | 172813 | 0.72 | 0.974294 |
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Target: 5'- ----aGCGCAUGCCGCUggaaacaAUCACAUCa -3' miRNA: 3'- cgcaaUGCGUAUGGUGGg------UAGUGUAG- -5' |
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| 17756 | 5' | -49.4 | NC_004629.1 | + | 13776 | 0.73 | 0.968617 |
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Target: 5'- uCGUUAUGgGUACCgACCCAUCugAa- -3' miRNA: 3'- cGCAAUGCgUAUGG-UGGGUAGugUag -5' |
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| 17756 | 5' | -49.4 | NC_004629.1 | + | 62767 | 0.74 | 0.941863 |
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Target: 5'- aGUGcUUGCGCAaauccACCACCCAUCgGCAUUu -3' miRNA: 3'- -CGC-AAUGCGUa----UGGUGGGUAG-UGUAG- -5' |
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| 17756 | 5' | -49.4 | NC_004629.1 | + | 189546 | 0.75 | 0.915669 |
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Target: 5'- aGCuUUugGCGUAUCGCCaaaaauauCAUCACAUCa -3' miRNA: 3'- -CGcAAugCGUAUGGUGG--------GUAGUGUAG- -5' |
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| 17756 | 5' | -49.4 | NC_004629.1 | + | 131975 | 1.12 | 0.015819 |
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Target: 5'- uGCGUUACGCAUACCACCCAUCACAUCc -3' miRNA: 3'- -CGCAAUGCGUAUGGUGGGUAGUGUAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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