Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
17757 | 5' | -45.9 | NC_004629.1 | + | 196258 | 0.67 | 0.999993 |
Target: 5'- -aACUugGCGUUCAU--CUGGAGUagGCAa -3' miRNA: 3'- gaUGA--CGUAAGUAuuGACCUCAa-CGU- -5' |
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17757 | 5' | -45.9 | NC_004629.1 | + | 146439 | 0.69 | 0.99994 |
Target: 5'- gCUACUgGCGUUCGUAGCc--AGUUGCu -3' miRNA: 3'- -GAUGA-CGUAAGUAUUGaccUCAACGu -5' |
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17757 | 5' | -45.9 | NC_004629.1 | + | 149328 | 0.73 | 0.995583 |
Target: 5'- -gGC-GCAUUCAUGGCcGGAGcUGCAc -3' miRNA: 3'- gaUGaCGUAAGUAUUGaCCUCaACGU- -5' |
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17757 | 5' | -45.9 | NC_004629.1 | + | 146713 | 1.1 | 0.036292 |
Target: 5'- uCUACUGCAUUCAUAACUGGAGUUGCAg -3' miRNA: 3'- -GAUGACGUAAGUAUUGACCUCAACGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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