Results 1 - 11 of 11 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 17758 | 3' | -56 | NC_004629.1 | + | 151661 | 0.67 | 0.945766 |
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Target: 5'- --cCACCUUGUuuACCcUCAGGGUCUu -3' miRNA: 3'- gaaGUGGAACGccUGGaAGUCCCGGG- -5' |
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| 17758 | 3' | -56 | NC_004629.1 | + | 150576 | 0.67 | 0.941432 |
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Target: 5'- gUUCAUUccGUGGuCCg-CAGGGCCCu -3' miRNA: 3'- gAAGUGGaaCGCCuGGaaGUCCCGGG- -5' |
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| 17758 | 3' | -56 | NC_004629.1 | + | 151424 | 0.68 | 0.927117 |
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Target: 5'- gUUUACCUUGCGGACCU-----GCCa -3' miRNA: 3'- gAAGUGGAACGCCUGGAaguccCGGg -5' |
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| 17758 | 3' | -56 | NC_004629.1 | + | 150080 | 0.68 | 0.915358 |
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Target: 5'- --aCACCacagggugaacGUGGACCUg-AGGGCCCu -3' miRNA: 3'- gaaGUGGaa---------CGCCUGGAagUCCCGGG- -5' |
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| 17758 | 3' | -56 | NC_004629.1 | + | 150683 | 0.68 | 0.910818 |
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Target: 5'- gUUCACCUUGUuuACCaUCGGGaCCCg -3' miRNA: 3'- gAAGUGGAACGccUGGaAGUCCcGGG- -5' |
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| 17758 | 3' | -56 | NC_004629.1 | + | 150447 | 0.73 | 0.691016 |
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Target: 5'- gUUCACCcUGaGGACCUUCAGGaCCa -3' miRNA: 3'- gAAGUGGaACgCCUGGAAGUCCcGGg -5' |
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| 17758 | 3' | -56 | NC_004629.1 | + | 151862 | 0.79 | 0.393031 |
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Target: 5'- cCUUCAgguCCUUGUGGGCCUUCuGGaCCCa -3' miRNA: 3'- -GAAGU---GGAACGCCUGGAAGuCCcGGG- -5' |
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| 17758 | 3' | -56 | NC_004629.1 | + | 151244 | 0.8 | 0.361509 |
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Target: 5'- ---gACCUUGUGGGCCUucuggacccUCGGGGCCUu -3' miRNA: 3'- gaagUGGAACGCCUGGA---------AGUCCCGGG- -5' |
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| 17758 | 3' | -56 | NC_004629.1 | + | 150638 | 0.9 | 0.086278 |
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Target: 5'- gUUUACCUUGUGGACCcUCAGGGCCUu -3' miRNA: 3'- gAAGUGGAACGCCUGGaAGUCCCGGG- -5' |
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| 17758 | 3' | -56 | NC_004629.1 | + | 150412 | 1.07 | 0.007094 |
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Target: 5'- uCUUUACCUUGCGGACCUUCAGGGCCCu -3' miRNA: 3'- -GAAGUGGAACGCCUGGAAGUCCCGGG- -5' |
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| 17758 | 3' | -56 | NC_004629.1 | + | 150900 | 1.11 | 0.003946 |
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Target: 5'- uCUUCACCUUGCGGACCUUCAGGGCCCu -3' miRNA: 3'- -GAAGUGGAACGCCUGGAAGUCCCGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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