miRNA: 3'- -UUAUgCCGGUAACGUCAAAggaGAUC- -5'
miRNA: 3'- -UUAUGCCGGUaACGUCAaagGAGAUC- -5'
miRNA: 3'- -UUAUGCCGGUAACGUCaaag-----GAGAUC- -5'
miRNA: 3'- -UUAUGCCGGUAACGUCAAAGGAGAUC- -5'
Results 1 - 4 of 4 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 17760 | 3' | -48.8 | NC_004629.1 | + | 175562 | 0.67 | 0.999447 |
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Target: 5'- aGAUG-GGCCAUUGUAGUUUag-CUGGu -3' miRNA: 3'- -UUAUgCCGGUAACGUCAAAggaGAUC- -5' |
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| 17760 | 3' | -48.8 | NC_004629.1 | + | 172911 | 0.69 | 0.997428 |
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Target: 5'- uAAUACGGUCA-UGCAGUauaCUUUAGg -3' miRNA: 3'- -UUAUGCCGGUaACGUCAaagGAGAUC- -5' |
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| 17760 | 3' | -48.8 | NC_004629.1 | + | 11884 | 0.76 | 0.878906 |
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Target: 5'- cGGUACGGCCAUUGCAGguguacuggCUUUAGg -3' miRNA: 3'- -UUAUGCCGGUAACGUCaaag-----GAGAUC- -5' |
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| 17760 | 3' | -48.8 | NC_004629.1 | + | 173254 | 1.08 | 0.024593 |
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Target: 5'- uAAUACGGCCAUUGCAGUUUCCUCUAGg -3' miRNA: 3'- -UUAUGCCGGUAACGUCAAAGGAGAUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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