Results 1 - 11 of 11 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
17761 | 5' | -45.2 | NC_004629.1 | + | 158340 | 0.66 | 1 |
Target: 5'- aGAGGGC-UUAUGCugUUAuc-CAAu -3' miRNA: 3'- cCUCCCGcAAUACGugAAUuauGUUu -5' |
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17761 | 5' | -45.2 | NC_004629.1 | + | 112268 | 0.67 | 1 |
Target: 5'- uGAGGGUcauaUGUGCACUUAugaGCAAc -3' miRNA: 3'- cCUCCCGca--AUACGUGAAUua-UGUUu -5' |
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17761 | 5' | -45.2 | NC_004629.1 | + | 277571 | 0.67 | 0.999998 |
Target: 5'- gGGAGGGUG-UAUaCACUUAaagaGUGCAc- -3' miRNA: 3'- -CCUCCCGCaAUAcGUGAAU----UAUGUuu -5' |
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17761 | 5' | -45.2 | NC_004629.1 | + | 207990 | 0.67 | 0.999998 |
Target: 5'- gGGAGGGC-UUAUGCcguuaaACUUu-UACAAGu -3' miRNA: 3'- -CCUCCCGcAAUACG------UGAAuuAUGUUU- -5' |
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17761 | 5' | -45.2 | NC_004629.1 | + | 85160 | 0.67 | 0.999998 |
Target: 5'- gGGAGGGCaUUAUGUugUauuuUACAu- -3' miRNA: 3'- -CCUCCCGcAAUACGugAauu-AUGUuu -5' |
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17761 | 5' | -45.2 | NC_004629.1 | + | 143068 | 0.68 | 0.999997 |
Target: 5'- uGGAGGGgauUUAUGCugUUAA-GCAAAu -3' miRNA: 3'- -CCUCCCgc-AAUACGugAAUUaUGUUU- -5' |
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17761 | 5' | -45.2 | NC_004629.1 | + | 120494 | 0.68 | 0.999991 |
Target: 5'- gGGAGGGCuUUAUGUugUau-UACAu- -3' miRNA: 3'- -CCUCCCGcAAUACGugAauuAUGUuu -5' |
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17761 | 5' | -45.2 | NC_004629.1 | + | 178418 | 0.71 | 0.999724 |
Target: 5'- gGGAGGGC-UUAUGCugUcauGUugGAAu -3' miRNA: 3'- -CCUCCCGcAAUACGugAau-UAugUUU- -5' |
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17761 | 5' | -45.2 | NC_004629.1 | + | 167959 | 0.73 | 0.998471 |
Target: 5'- gGGAGGGC-UUAUGCugUUaAAUACc-- -3' miRNA: 3'- -CCUCCCGcAAUACGugAA-UUAUGuuu -5' |
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17761 | 5' | -45.2 | NC_004629.1 | + | 25476 | 0.75 | 0.993126 |
Target: 5'- gGGAGGGC-UUAUGCugUUAAguaACAu- -3' miRNA: 3'- -CCUCCCGcAAUACGugAAUUa--UGUuu -5' |
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17761 | 5' | -45.2 | NC_004629.1 | + | 173915 | 1.1 | 0.047393 |
Target: 5'- gGGAGGGCGUUAUGCACUUAAUACAAAu -3' miRNA: 3'- -CCUCCCGCAAUACGUGAAUUAUGUUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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