Results 1 - 5 of 5 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
17762 | 3' | -52.8 | NC_004629.1 | + | 171610 | 0.66 | 0.995842 |
Target: 5'- aUGCACCGuGUGUUgauuCAAagUGGCUGa -3' miRNA: 3'- gGCGUGGC-CAUAGau--GUUg-ACCGACa -5' |
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17762 | 3' | -52.8 | NC_004629.1 | + | 201615 | 0.66 | 0.995181 |
Target: 5'- aUCGCgAUUGGUAUCUACAacaauugaACUaGGUUGa -3' miRNA: 3'- -GGCG-UGGCCAUAGAUGU--------UGA-CCGACa -5' |
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17762 | 3' | -52.8 | NC_004629.1 | + | 194880 | 0.67 | 0.990482 |
Target: 5'- -gGCACCaaccucAUCaGCAGCUGGCUGg -3' miRNA: 3'- ggCGUGGcca---UAGaUGUUGACCGACa -5' |
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17762 | 3' | -52.8 | NC_004629.1 | + | 45700 | 0.7 | 0.946428 |
Target: 5'- gCGCGCCuGG-GUCUACuACUGGCa-- -3' miRNA: 3'- gGCGUGG-CCaUAGAUGuUGACCGaca -5' |
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17762 | 3' | -52.8 | NC_004629.1 | + | 195872 | 1.09 | 0.011116 |
Target: 5'- gCCGCACCGGUAUCUACAACUGGCUGUu -3' miRNA: 3'- -GGCGUGGCCAUAGAUGUUGACCGACA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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