Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 17763 | 5' | -48.9 | NC_004629.1 | + | 149691 | 0.66 | 0.999939 |
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Target: 5'- gCCAuCCUUgaugguguaGGUGUCaACUUGAUuuGCAUCg -3' miRNA: 3'- -GGU-GGAA---------UCAUAG-UGGACUGu-CGUAG- -5' |
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| 17763 | 5' | -48.9 | NC_004629.1 | + | 46086 | 0.66 | 0.999906 |
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Target: 5'- uUACUUUAGUgaauacuuguagggcAUCugCUGAacgAGCAUCa -3' miRNA: 3'- gGUGGAAUCA---------------UAGugGACUg--UCGUAG- -5' |
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| 17763 | 5' | -48.9 | NC_004629.1 | + | 198810 | 0.66 | 0.999896 |
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Target: 5'- cCCAUUUacaugauaAGUAUCACCUGGuuCAGgGUCa -3' miRNA: 3'- -GGUGGAa-------UCAUAGUGGACU--GUCgUAG- -5' |
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| 17763 | 5' | -48.9 | NC_004629.1 | + | 112994 | 0.67 | 0.999723 |
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Target: 5'- gCCACCgggUGGUuUCA--UGACAGCAa- -3' miRNA: 3'- -GGUGGa--AUCAuAGUggACUGUCGUag -5' |
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| 17763 | 5' | -48.9 | NC_004629.1 | + | 127822 | 0.67 | 0.999566 |
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Target: 5'- aCCACaggUAGUAauUCACCUGGCuuauuaucucuGCAUg -3' miRNA: 3'- -GGUGga-AUCAU--AGUGGACUGu----------CGUAg -5' |
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| 17763 | 5' | -48.9 | NC_004629.1 | + | 54463 | 0.68 | 0.998798 |
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Target: 5'- aCUGgUUUAGUAUCACCaugGAUAGC-UCg -3' miRNA: 3'- -GGUgGAAUCAUAGUGGa--CUGUCGuAG- -5' |
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| 17763 | 5' | -48.9 | NC_004629.1 | + | 156459 | 0.7 | 0.993726 |
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Target: 5'- uUACUUUAGaAUCACC-GAUAGCAUUu -3' miRNA: 3'- gGUGGAAUCaUAGUGGaCUGUCGUAG- -5' |
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| 17763 | 5' | -48.9 | NC_004629.1 | + | 232063 | 1.12 | 0.017326 |
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Target: 5'- uCCACCUUAGUAUCACCUGACAGCAUCc -3' miRNA: 3'- -GGUGGAAUCAUAGUGGACUGUCGUAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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