Results 1 - 12 of 12 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
17764 | 5' | -45.8 | NC_004629.1 | + | 35363 | 0.66 | 1 |
Target: 5'- uGCUGAguUaGGUGUAUU-CCGUGAUa -3' miRNA: 3'- gUGACUguA-CUACAUGAuGGCAUUGg -5' |
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17764 | 5' | -45.8 | NC_004629.1 | + | 65764 | 0.66 | 1 |
Target: 5'- cCACUcGCuUGAgUGUACUcCgCGUAACCg -3' miRNA: 3'- -GUGAcUGuACU-ACAUGAuG-GCAUUGG- -5' |
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17764 | 5' | -45.8 | NC_004629.1 | + | 190627 | 0.67 | 0.999999 |
Target: 5'- gACUGGuCGUGAUGaUAUUACaCGUGAa- -3' miRNA: 3'- gUGACU-GUACUAC-AUGAUG-GCAUUgg -5' |
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17764 | 5' | -45.8 | NC_004629.1 | + | 37890 | 0.68 | 0.999996 |
Target: 5'- --aUGACAUcauuaGAUGUGCUACCugauGCCc -3' miRNA: 3'- gugACUGUA-----CUACAUGAUGGcau-UGG- -5' |
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17764 | 5' | -45.8 | NC_004629.1 | + | 138776 | 0.68 | 0.999994 |
Target: 5'- aCAUUGAUGcucaGAUGUGCggugguauaUACUGUAACCu -3' miRNA: 3'- -GUGACUGUa---CUACAUG---------AUGGCAUUGG- -5' |
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17764 | 5' | -45.8 | NC_004629.1 | + | 4859 | 0.68 | 0.999987 |
Target: 5'- gCACUGACGUaGAugauguucgUGUACUGgucggugaagccuuCCGUAACg -3' miRNA: 3'- -GUGACUGUA-CU---------ACAUGAU--------------GGCAUUGg -5' |
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17764 | 5' | -45.8 | NC_004629.1 | + | 100895 | 0.69 | 0.999984 |
Target: 5'- aACUGGugaAUGggGUGCUACCGguacuGCUa -3' miRNA: 3'- gUGACUg--UACuaCAUGAUGGCau---UGG- -5' |
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17764 | 5' | -45.8 | NC_004629.1 | + | 239627 | 0.69 | 0.999971 |
Target: 5'- gCAUgguuACAUGAUGUaauugaggauACUGCUGUAACUg -3' miRNA: 3'- -GUGac--UGUACUACA----------UGAUGGCAUUGG- -5' |
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17764 | 5' | -45.8 | NC_004629.1 | + | 216256 | 0.7 | 0.999929 |
Target: 5'- aGCUGAUcaagAUGAUGUACUuggccacGCCGguuUGACUa -3' miRNA: 3'- gUGACUG----UACUACAUGA-------UGGC---AUUGG- -5' |
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17764 | 5' | -45.8 | NC_004629.1 | + | 227737 | 0.71 | 0.999608 |
Target: 5'- aCGCUGACGUGcuu--CUGCUGUGGCa -3' miRNA: 3'- -GUGACUGUACuacauGAUGGCAUUGg -5' |
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17764 | 5' | -45.8 | NC_004629.1 | + | 149274 | 0.72 | 0.999394 |
Target: 5'- aACUGAUAUug-GUACUGaaGUAACCa -3' miRNA: 3'- gUGACUGUAcuaCAUGAUggCAUUGG- -5' |
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17764 | 5' | -45.8 | NC_004629.1 | + | 11691 | 1.15 | 0.026897 |
Target: 5'- uCACUGACAUGAUGUACUACCGUAACCa -3' miRNA: 3'- -GUGACUGUACUACAUGAUGGCAUUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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