miRNA: 3'- aCGAaUGGGUAGACuucuACC-AGUag -5'
miRNA: 3'- acgAAUGGGu-AGACUUCU--ACCa--GUAG- -5'
miRNA: 3'- -ACGAa---UGGGUAGACU------------UCUACCAGUag -5'
miRNA: 3'- -ACGAAUGGGUAGACUUCUACCAGUAG- -5'
Results 1 - 4 of 4 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 17765 | 3' | -48.8 | NC_004629.1 | + | 182810 | 0.66 | 0.999902 |
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Target: 5'- aGCUgGCCCAUCUGucuuUGGaUCAa- -3' miRNA: 3'- aCGAaUGGGUAGACuucuACC-AGUag -5' |
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| 17765 | 3' | -48.8 | NC_004629.1 | + | 181563 | 0.66 | 0.999873 |
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Target: 5'- --gUUGCCCucUCUGAAGAgcUGGaagCAUCu -3' miRNA: 3'- acgAAUGGGu-AGACUUCU--ACCa--GUAG- -5' |
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| 17765 | 3' | -48.8 | NC_004629.1 | + | 91034 | 0.73 | 0.969911 |
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Target: 5'- aUGCUguacGCCCAUUUGGucguaauuacucAGGUGGUCAa- -3' miRNA: 3'- -ACGAa---UGGGUAGACU------------UCUACCAGUag -5' |
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| 17765 | 3' | -48.8 | NC_004629.1 | + | 53101 | 1.11 | 0.017358 |
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Target: 5'- aUGCUUACCCAUCUGAAGAUGGUCAUCu -3' miRNA: 3'- -ACGAAUGGGUAGACUUCUACCAGUAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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