miRNA: 3'- gUCAUCCCGa--UAUACggCAaCUCAUU- -5'
miRNA: 3'- gUCAUCCCGauaUACGGCA--------------ACUCauu -5'
miRNA: 3'- gUCaUCCCGAuAUACGGCAAcUCAUU- -5'
miRNA: 3'- -GUCaUCCCGAUAUACGGCAacUCAuu -5'
miRNA: 3'- -GUCAUCCCGAUAUACGGCAACUCAUU- -5'
Results 1 - 5 of 5 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 17767 | 5' | -49.5 | NC_004629.1 | + | 235302 | 0.66 | 0.999688 |
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Target: 5'- gAGUAGGGUaaaGUAUGuaGUaGAGUAAa -3' miRNA: 3'- gUCAUCCCGa--UAUACggCAaCUCAUU- -5' |
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| 17767 | 5' | -49.5 | NC_004629.1 | + | 273853 | 0.66 | 0.999666 |
|
Target: 5'- uGGUAGGGCaa-GUGCCGUuuuaagaagucuagUGAGc-- -3' miRNA: 3'- gUCAUCCCGauaUACGGCA--------------ACUCauu -5' |
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| 17767 | 5' | -49.5 | NC_004629.1 | + | 25477 | 0.66 | 0.999511 |
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Target: 5'- uGGgAGGGCU-UAUGCUGUUaAGUAAc -3' miRNA: 3'- gUCaUCCCGAuAUACGGCAAcUCAUU- -5' |
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| 17767 | 5' | -49.5 | NC_004629.1 | + | 12175 | 0.69 | 0.99302 |
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Target: 5'- aCAGgAGGGCUAUAUGUCGUcaAGUu- -3' miRNA: 3'- -GUCaUCCCGAUAUACGGCAacUCAuu -5' |
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| 17767 | 5' | -49.5 | NC_004629.1 | + | 114172 | 1.07 | 0.024714 |
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Target: 5'- gCAGUAGGGCUAUAUGCCGUUGAGUAAa -3' miRNA: 3'- -GUCAUCCCGAUAUACGGCAACUCAUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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