Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 17768 | 3' | -42.9 | NC_004629.1 | + | 30538 | 0.66 | 1 |
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Target: 5'- -cUAGCGGCUGGUGccguuAUUUGcGGUAa -3' miRNA: 3'- uuGUCGUCGACUAUaau--UAAAU-CCAU- -5' |
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| 17768 | 3' | -42.9 | NC_004629.1 | + | 193072 | 0.68 | 1 |
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Target: 5'- cAACAGCuGCUGcAUAUgcUAGUUUuGGUGg -3' miRNA: 3'- -UUGUCGuCGAC-UAUA--AUUAAAuCCAU- -5' |
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| 17768 | 3' | -42.9 | NC_004629.1 | + | 76192 | 0.68 | 1 |
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Target: 5'- cAGCGGCAGCaGGUGUUGGU---GGUc -3' miRNA: 3'- -UUGUCGUCGaCUAUAAUUAaauCCAu -5' |
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| 17768 | 3' | -42.9 | NC_004629.1 | + | 183011 | 0.67 | 1 |
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Target: 5'- cAGCAGCAGCaaGGUAUUuAUgcugaUGGGUAu -3' miRNA: 3'- -UUGUCGUCGa-CUAUAAuUAa----AUCCAU- -5' |
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| 17768 | 3' | -42.9 | NC_004629.1 | + | 121443 | 0.66 | 1 |
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Target: 5'- cAUGGCGGUUGGauucagAGUUUAGGUAu -3' miRNA: 3'- uUGUCGUCGACUauaa--UUAAAUCCAU- -5' |
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| 17768 | 3' | -42.9 | NC_004629.1 | + | 96411 | 0.7 | 0.999997 |
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Target: 5'- uACAGCAGUgcaaGAUAUUAAUcaAGGUc -3' miRNA: 3'- uUGUCGUCGa---CUAUAAUUAaaUCCAu -5' |
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| 17768 | 3' | -42.9 | NC_004629.1 | + | 182805 | 0.72 | 0.999909 |
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Target: 5'- cACAGCAGCUGGUGUUAAc------- -3' miRNA: 3'- uUGUCGUCGACUAUAAUUaaauccau -5' |
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| 17768 | 3' | -42.9 | NC_004629.1 | + | 135457 | 0.74 | 0.999455 |
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Target: 5'- cGCAGUAGUUGGUGUUGcagcUGGGUAg -3' miRNA: 3'- uUGUCGUCGACUAUAAUuaa-AUCCAU- -5' |
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| 17768 | 3' | -42.9 | NC_004629.1 | + | 121884 | 0.84 | 0.876364 |
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Target: 5'- uAACAGCAGCUGAUAUUAAggaaAGGg- -3' miRNA: 3'- -UUGUCGUCGACUAUAAUUaaa-UCCau -5' |
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| 17768 | 3' | -42.9 | NC_004629.1 | + | 135113 | 1.06 | 0.109231 |
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Target: 5'- uAACAGCAGCUGAUAUUAAUUUAGGUAa -3' miRNA: 3'- -UUGUCGUCGACUAUAAUUAAAUCCAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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