Results 1 - 6 of 6 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
17769 | 3' | -63.4 | NC_004629.1 | + | 151331 | 0.67 | 0.646727 |
Target: 5'- aGGGUaUCC-AGGGUgUcGAAGGCCCACa -3' miRNA: 3'- -UCCG-GGGcUCCCAgGuCUUCCGGGUG- -5' |
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17769 | 3' | -63.4 | NC_004629.1 | + | 150586 | 0.69 | 0.534556 |
Target: 5'- uGGUCCgcAGGGcCCuGAAGGUCCGCa -3' miRNA: 3'- uCCGGGgcUCCCaGGuCUUCCGGGUG- -5' |
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17769 | 3' | -63.4 | NC_004629.1 | + | 151538 | 0.75 | 0.239689 |
Target: 5'- uAGGUgUUuuagcaauGGGUCCAGAAGGCCCACa -3' miRNA: 3'- -UCCGgGGcu------CCCAGGUCUUCCGGGUG- -5' |
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17769 | 3' | -63.4 | NC_004629.1 | + | 150097 | 0.75 | 0.239689 |
Target: 5'- uGGaCCUGAGGGcCCuGAAGGUCCGCa -3' miRNA: 3'- uCCgGGGCUCCCaGGuCUUCCGGGUG- -5' |
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17769 | 3' | -63.4 | NC_004629.1 | + | 150333 | 0.79 | 0.134356 |
Target: 5'- aAGGCCCUGAGGGUCCAcAAGGUaaaCCAg -3' miRNA: 3'- -UCCGGGGCUCCCAGGUcUUCCG---GGUg -5' |
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17769 | 3' | -63.4 | NC_004629.1 | + | 150932 | 1.09 | 0.001189 |
Target: 5'- aAGGCCCCGAGGGUCCAGAAGGCCCACa -3' miRNA: 3'- -UCCGGGGCUCCCAGGUCUUCCGGGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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