Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 17769 | 5' | -50.3 | NC_004629.1 | + | 239128 | 0.66 | 0.999537 |
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Target: 5'- -aGUGaAGCUUGUCCAGAcCAUUGCc- -3' miRNA: 3'- acCAC-UUGGAUAGGUCUaGUAGCGuc -5' |
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| 17769 | 5' | -50.3 | NC_004629.1 | + | 13633 | 0.67 | 0.998088 |
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Target: 5'- gGGUGAACCUucaccaccaacuGUCCAGAUauugaagaagucccUAUCgGCAa -3' miRNA: 3'- aCCACUUGGA------------UAGGUCUA--------------GUAG-CGUc -5' |
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| 17769 | 5' | -50.3 | NC_004629.1 | + | 151322 | 0.68 | 0.995867 |
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Target: 5'- aUGGUGAGCagggUAUCCAGggUGUCGaAGg -3' miRNA: 3'- -ACCACUUGg---AUAGGUCuaGUAGCgUC- -5' |
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| 17769 | 5' | -50.3 | NC_004629.1 | + | 52034 | 0.69 | 0.991542 |
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Target: 5'- gGGUGAGCgUAgCCAGG-CAUUGCGu -3' miRNA: 3'- aCCACUUGgAUaGGUCUaGUAGCGUc -5' |
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| 17769 | 5' | -50.3 | NC_004629.1 | + | 53822 | 0.7 | 0.987582 |
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Target: 5'- aGGUaAACCUGUUCAGAUCGguaagcUCGUAc -3' miRNA: 3'- aCCAcUUGGAUAGGUCUAGU------AGCGUc -5' |
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| 17769 | 5' | -50.3 | NC_004629.1 | + | 150397 | 0.7 | 0.987582 |
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Target: 5'- aGGUGAACCUgGUCCAcAagGUCGUAa -3' miRNA: 3'- aCCACUUGGA-UAGGUcUagUAGCGUc -5' |
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| 17769 | 5' | -50.3 | NC_004629.1 | + | 150968 | 1.09 | 0.018637 |
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Target: 5'- cUGGUGAACCUAUCCAGAUCAUCGCAGc -3' miRNA: 3'- -ACCACUUGGAUAGGUCUAGUAGCGUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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