Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 17773 | 5' | -49.8 | NC_004629.1 | + | 230238 | 0.66 | 0.999838 |
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Target: 5'- aUAACAGUgAGUUuUGGuGUACCGGUa- -3' miRNA: 3'- -GUUGUCGgUCAAcAUC-UAUGGCCAcg -5' |
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| 17773 | 5' | -49.8 | NC_004629.1 | + | 124692 | 0.66 | 0.999789 |
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Target: 5'- aUggUAGCCGGUUGaccauaggucuguUGGAUACC--UGCg -3' miRNA: 3'- -GuuGUCGGUCAAC-------------AUCUAUGGccACG- -5' |
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| 17773 | 5' | -49.8 | NC_004629.1 | + | 4934 | 0.67 | 0.999065 |
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Target: 5'- --cUAGCCuGGUUaacGUAGAugcuguUACCGGUGUa -3' miRNA: 3'- guuGUCGG-UCAA---CAUCU------AUGGCCACG- -5' |
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| 17773 | 5' | -49.8 | NC_004629.1 | + | 91338 | 0.67 | 0.998866 |
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Target: 5'- cCAAUGGCCAauGUuacuagcuacUGaaccAGAUGCUGGUGCa -3' miRNA: 3'- -GUUGUCGGU--CA----------ACa---UCUAUGGCCACG- -5' |
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| 17773 | 5' | -49.8 | NC_004629.1 | + | 168040 | 0.67 | 0.998866 |
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Target: 5'- -uAUGGCUAGUUGUGGAcacacagcUACUGGUa- -3' miRNA: 3'- guUGUCGGUCAACAUCU--------AUGGCCAcg -5' |
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| 17773 | 5' | -49.8 | NC_004629.1 | + | 193071 | 0.7 | 0.988373 |
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Target: 5'- uCAACAGCUgcugcauaugcuAGUuuugGUGG-UACUGGUGCa -3' miRNA: 3'- -GUUGUCGG------------UCAa---CAUCuAUGGCCACG- -5' |
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| 17773 | 5' | -49.8 | NC_004629.1 | + | 47675 | 0.72 | 0.976834 |
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Target: 5'- aAACAGCCA--UGUuacccAUACCGGUGUa -3' miRNA: 3'- gUUGUCGGUcaACAuc---UAUGGCCACG- -5' |
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| 17773 | 5' | -49.8 | NC_004629.1 | + | 195564 | 1.13 | 0.011327 |
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Target: 5'- uCAACAGCCAGUUGUAGAUACCGGUGCg -3' miRNA: 3'- -GUUGUCGGUCAACAUCUAUGGCCACG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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