Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 17775 | 5' | -47.2 | NC_004629.1 | + | 110245 | 0.66 | 0.999996 |
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Target: 5'- cAGGACAUCGUCGcugguuUUAAaguaGCGCCu -3' miRNA: 3'- -UUCUGUAGUAGCu-----AGUUgaagCGUGG- -5' |
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| 17775 | 5' | -47.2 | NC_004629.1 | + | 90079 | 0.66 | 0.999994 |
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Target: 5'- uGAGA-AUUAUUGAUUAugUacgugaacUCGCACCa -3' miRNA: 3'- -UUCUgUAGUAGCUAGUugA--------AGCGUGG- -5' |
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| 17775 | 5' | -47.2 | NC_004629.1 | + | 89652 | 0.66 | 0.999994 |
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Target: 5'- cAAGAUAUgAUCGAUgAACauuaUCGCAUUc -3' miRNA: 3'- -UUCUGUAgUAGCUAgUUGa---AGCGUGG- -5' |
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| 17775 | 5' | -47.2 | NC_004629.1 | + | 101343 | 0.67 | 0.999984 |
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Target: 5'- aGAGAUAUCA-CGGUUAGCUUgGUcaagacgacguacACCg -3' miRNA: 3'- -UUCUGUAGUaGCUAGUUGAAgCG-------------UGG- -5' |
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| 17775 | 5' | -47.2 | NC_004629.1 | + | 155735 | 0.68 | 0.999887 |
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Target: 5'- uAGACAuaacuguuaaaUCAggugCGuAUCAAC-UCGCACCu -3' miRNA: 3'- uUCUGU-----------AGUa---GC-UAGUUGaAGCGUGG- -5' |
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| 17775 | 5' | -47.2 | NC_004629.1 | + | 268207 | 0.71 | 0.998068 |
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Target: 5'- -cGACAUcCAUUGAccagcuucaguaaUCAGCUUUGCACg -3' miRNA: 3'- uuCUGUA-GUAGCU-------------AGUUGAAGCGUGg -5' |
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| 17775 | 5' | -47.2 | NC_004629.1 | + | 254006 | 0.72 | 0.995629 |
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Target: 5'- uGAGACAUCgcauaGUCGAcuacaUCAACaUCGcCGCCa -3' miRNA: 3'- -UUCUGUAG-----UAGCU-----AGUUGaAGC-GUGG- -5' |
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| 17775 | 5' | -47.2 | NC_004629.1 | + | 273269 | 0.78 | 0.912915 |
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Target: 5'- uAGACA---UCGGUC-ACUUCGCACCa -3' miRNA: 3'- uUCUGUaguAGCUAGuUGAAGCGUGG- -5' |
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| 17775 | 5' | -47.2 | NC_004629.1 | + | 232364 | 1.12 | 0.024226 |
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Target: 5'- cAAGACAUCAUCGAUCAACUUCGCACCa -3' miRNA: 3'- -UUCUGUAGUAGCUAGUUGAAGCGUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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