Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 17777 | 5' | -42.7 | NC_004629.1 | + | 146201 | 0.67 | 1 |
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Target: 5'- -aACAacaUCCGUAgUUGcauuGAUUGGUGUUg -3' miRNA: 3'- gaUGUg--AGGCAUaAAU----UUAACCACGG- -5' |
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| 17777 | 5' | -42.7 | NC_004629.1 | + | 254978 | 0.66 | 1 |
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Target: 5'- -aACACUCCGUGU---AGUacGUGUCc -3' miRNA: 3'- gaUGUGAGGCAUAaauUUAacCACGG- -5' |
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| 17777 | 5' | -42.7 | NC_004629.1 | + | 79658 | 0.66 | 1 |
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Target: 5'- -aACACcaucgCCGUG---AAGUUGGUGUa -3' miRNA: 3'- gaUGUGa----GGCAUaaaUUUAACCACGg -5' |
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| 17777 | 5' | -42.7 | NC_004629.1 | + | 261348 | 0.66 | 1 |
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Target: 5'- -gACACaUCCGUAUcguUUAAAUaGGUGa- -3' miRNA: 3'- gaUGUG-AGGCAUA---AAUUUAaCCACgg -5' |
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| 17777 | 5' | -42.7 | NC_004629.1 | + | 50300 | 0.66 | 1 |
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Target: 5'- aUACACUUugguaGUGUUcAAGaUGGUGUCa -3' miRNA: 3'- gAUGUGAGg----CAUAAaUUUaACCACGG- -5' |
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| 17777 | 5' | -42.7 | NC_004629.1 | + | 45106 | 0.68 | 1 |
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Target: 5'- -aAUGCUCCGUcacgUAAAUUGGgGCa -3' miRNA: 3'- gaUGUGAGGCAuaa-AUUUAACCaCGg -5' |
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| 17777 | 5' | -42.7 | NC_004629.1 | + | 187087 | 0.69 | 1 |
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Target: 5'- aCUGCGC-CCGag---AAAUUGGUGUa -3' miRNA: 3'- -GAUGUGaGGCauaaaUUUAACCACGg -5' |
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| 17777 | 5' | -42.7 | NC_004629.1 | + | 253422 | 0.72 | 0.999997 |
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Target: 5'- aCUGCugUCCagcUGUUaaaUAAGUUGG-GCCa -3' miRNA: 3'- -GAUGugAGGc--AUAA---AUUUAACCaCGG- -5' |
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| 17777 | 5' | -42.7 | NC_004629.1 | + | 165006 | 0.75 | 0.999813 |
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Target: 5'- uCUGCuGCUUCGUGUUguug-UGGUGUCa -3' miRNA: 3'- -GAUG-UGAGGCAUAAauuuaACCACGG- -5' |
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| 17777 | 5' | -42.7 | NC_004629.1 | + | 273063 | 1.14 | 0.058181 |
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Target: 5'- gCUACACUCCGUAUUUAAAUUGGUGCCc -3' miRNA: 3'- -GAUGUGAGGCAUAAAUUUAACCACGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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